糖尿病大鼠心肌组织microRNA-27b的改变及意义  

The expression changes of microRNA-27b in the cardiac muscle of diabetic rats and its significance

作　　者：来丹丹[1] 钱正明[1] 黄建振[1] 李建芳[2] 

机构地区：[1]杭州市萧山区第一人民医院心内科,311200 [2]杭州市萧山区第一人民医院胃肠外科,311200

出　　处：《心电与循环》2015年第1期31-32,65,I0001,共4页Journal of Electrocardiology and Circulation

基　　金：杭州市卫生科技计划一般项目(项目编号:2011B050)

摘　　要：目的研究microRNA-27b在糖尿病大鼠心肌组织中的表达变化和意义。方法建立糖尿病大鼠模型,VG染色观察心肌组织形态及胶原容量,microRNA芯片筛选心肌纤维化相关microRNA,realtime PCR验证rrmicroRNA的表达改变。结果 VG染色显示糖尿病组大鼠心肌组织内胶原容量高于对照组(P<0.05),microRNA芯片提示糖尿病组大鼠与对照组相比心肌组织中多个microRNA出现表达差异,Realtime PCR证实糖尿病大鼠心肌组织中microRNA-27b的表达较对照组上调(P<0.05)。结论 microRNA-27b可能与糖尿病大鼠心肌纤维化的发生相关。Objective To investigate the expression changes of microRNA- 27b in the cardiac muscle of diabetic rats and its significance. Methods After establishment of diabetic rat model, van Gieson stain was used to determine myocardial morphology and collagen volume, microRNA chips were used to screen the microRNAs associated with myocardial fibrosis, and realtime PCR was performed to validate the expression changes of microRNA. Results Van Gieson stain displayed that the collagen volume of cardiac muscle in the diabetic rats was higher than that in control group （P 〈0.05）. MicroRNA chips uncovered abnormal expression of several microRNAs and realtime PCR validated up- regulation of microRNA- 27b expression in cardiac muscle of diabetic rats compared with control group （P〈0.05）. Conclusion MicroRNA- 27b may be associated with myocardial fibrosis of diabetic rats.

关 键 词：心肌纤维化 microRNA-27b 糖尿病 大鼠 

分 类 号：R587.2[医药卫生—内分泌]