绵羊皮肤鸟苷酸环化酶完整CDS区结构域分析  

Analysis of the domain of full CDS region of Guanylate Cyclase from sheep skin

作　　者：杨姗姗[1] 姬凯元[1] 石占全[1] 焦丁兴 白俊明[1] 范瑞文[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]河北省邢台市清河县农业局,河北清河054000

出　　处：《中国兽医杂志》2015年第2期30-32,50,共3页Chinese Journal of Veterinary Medicine

基　　金：山西省科技攻关项目(20120311024-2)

摘　　要：用RT-PCR法获得了绵羊皮肤的鸟苷酸环化酶完整CDS区,其长度为1 860 bp,与其他哺乳动物相比,高度保守。通过对其结构域分析,发现绵羊鸟氨酸环化酶(GC)编码区存在环化酶同源性结构域(CHD)、血红素一氧化氮结合位点(HNOB)和相关血红素一氧化氮结合位点(HNOBA),这些结构域分别发挥其催化功能以及在细胞内转导下游信号的功能。The full CDS region of guanylate cyclase （GC）from sheep skin was obtained by RT-PCR. The size of CDS of GCwas 1 860 bp, which showed high homology with that of other mammalians and so it was highly conservative highly. The domain offull CDS region of GC was analyzed by prediction.The results showed that amino acids contained cyclase homology domain（ CHD ）,which played roles in catalytic mechanism of the protein . hemo NO binding sites （HNOB）and hemo NO binding sites associated（HNOBA）, which participated in the transduction of the downstream signals in cells.

关 键 词：鸟苷酸环化酶 皮肤 结构域 绵羊 

分 类 号：S826[农业科学—畜牧学]