显脉旋覆花根中总黄酮提取方法的研究  

Study on the extraction method of the total flavonoids in the roots of Inula nervosa

作　　者：张虹[1] 李树伟[1] 李春燕[1] 袁寒[1] 白红丽[2] 郭俊明[2] 

机构地区：[1]红河学院云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [2]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2015年第2期112-114,共3页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：民族药资源化学国家民委-教育部重点实验室开放基金(MZY1405)

摘　　要：以显脉旋覆花根为试验材料,用乙醇和甲醇为提取剂,采用浸提法分别对显脉旋覆花根中总黄酮进行提取,并用紫外分光光度法进行了测定.结果表明:乙醇70%,甲醇体积分数50%,浸提时间均为24 h时,总黄酮的提取效果较好,乙醇和甲醇提取的总黄酮得率分别为2.33%和1.84%,乙醇的提取效果优于甲醇,总黄酮测定方法平均回收率为99.89%,相对标准偏差RSD为0.89%,结果可靠.In this test,the roots of Inula nervosa were used as the test materials,and their total flavonoids were extracted by alcohol-and methanol-based cold-maceration. The content of the extracted total flavonoids was determined with UV spectrophotometer. The results were the following： while alcohol was 70% and methanol 50%,and the extracting time was 24 h,the extraction of the total flavonoids had a good effect. The extraction rate of alcohol and methanol was 2. 33% and 1. 84% respectively. Alcohol had a better effect than methanol. The average recovery of this method was 99. 89%,and the relative standard deviation（ RSD） of the method was 0. 89%. Its results are reliable.

关 键 词：显脉旋覆花根 总黄酮 提取 

分 类 号：R284.2[医药卫生—中药学]