血红素氧合酶-1在大鼠内毒素性急性肺损伤中的作用  

Role of heme oxygenase-1 in endotoxin-induced lung injury in rats

作　　者：邬娇 郭曲练[2] 张重[2] 叶治[2] 赵双平[2] 

机构地区：[1]湖南省肿瘤医院(中南大学湘雅医学院附属肿瘤医院)麻醉科,湖南长沙410013 [2]中南大学湘雅医院麻醉科,湖南长沙410008

出　　处：《中国现代医学杂志》2015年第5期14-17,共4页China Journal of Modern Medicine

基　　金：湖南省科技厅基金(No:2013FJ4106);湖南省发改委基金(No:湘发改高技2012-1493)

摘　　要：目的探讨血红素氧合酶-1（HO-1）在大鼠内毒素性急性肺损伤（ALI）中的作用。方法健康雄性SD大鼠32只,体重220~250 g,随机分为4组（n=8）：对照组（C组）、锌原卟啉-IX（Zn PP-IX）预处理组（Z组）、Zn PP-IX预处理＋脂多糖（LPS）组（ZL组）和内毒素组（L组）。Z组和ZL组经尾静脉注射特异性HO-1拮抗剂Zn PP-IX 10μmol/kg,C组和L组分别注射等体积生理盐水。10 min后,L组和ZL组经尾静脉注射LPS 5 mg/kg;C组和Z组分别经尾静脉注射等体积生理盐水。给予LPS或生理盐水6 h后,心脏放血处死大鼠,取肺组织计算湿干重比（W/D）;采用弥漫性肺泡损伤（DAD）评分评价肺组织损伤程度;测定肺组织髓过氧化物酶（MPO）活性,丙二醛（MDA）含量,超氧化物歧化酶（SOD）活性以及HO-1 m RNA和HO-1蛋白表达水平。结果与C组比较,L组和ZL组肺组织W/D比值、DAD评分、MPO活性和MDA含量升高,SOD活性减弱,肺组织HO-1 m RNA和HO-1蛋白表达水平升高（P〈0.05）;Z组上述指标差异无统计学意义（P〉0.05）;与L组比较,ZL组肺组织W/D比值、DAD评分、MPO活性和MDA含量升高,SOD活性减弱,肺组织HO-1m RNA和HO-1蛋白表达水平降低（P〈0.05）。结论肺组织HO-1对大鼠内毒素性ALI可产生一定的保护作用。【Objective】 To investigate the role of heme oxygenase-1（HO-1） in endotoxin-induced lung injury in rats. 【Methods】 Thirty-two male SD rats weighing 220 ~ 250 g were randomly divided into 4 groups（n =8）： control group（group C）, Zn PP-IX group（group Z）, Zn PP- IX ＋ LPS group（group ZL） and LPS group（group L）. In the group Z and the group ZL, Zn PP- IX 10 μmol/ kg was given, isometric saline was given in the group C and the group L. Ten minutes later, LPS 5 mg/kg was given in the group L and the group ZL.The group C and the group Z respectively received isometric saline. The animals were killed at 6 h after LPS or normal saline administration. The lungs were removed for determination of W/D lung weight ratio, myeloperoxidase（MPO） activity, MDA content, SOD activity, expression of HO-1 and HO-1 m RNA. The severity of lung injury was evaluated using diffuse alveolar damage（DAD） score. 【Results】 Compared with the group C,W/D lung weight ratio, DAD score, MPO activity and MDA content were significantly increased, SOD activity was decreased, the expression of HO-1 and HO-1 m RNA up-regulated in the group L and the group ZL,while there was no significant difference in the above indices in the group Z. Compared with the group L, W/D lung weight ratio, DAD score, MPO activity and MDA content were significantly increased, SOD activity was decreased, while the expression of HO-1 and HO-1 m RNA down-regulated in the group ZL.【Conclusion】 HO-1 has protective effects on the lungs against endotoxin-induced acute lung injury in rats.

关 键 词：内毒素血症 血红素氧合酶 超氧化物歧化酶 

分 类 号：R614[医药卫生—麻醉学]