HPLC-ELSD测定得力生注射液的黄芪甲苷的含量  

Determination of content of Astragaloside A in Delisheng Injection by HPLC-ELSD

作　　者：罗丹[1] 罗国安[2] 陈丽[2] 马晋芳[2] 王义明[1] 

机构地区：[1]江西中医学院,现代中药制剂教育部重点实验室,江西南昌330004 [2]清华大学,北京100084

出　　处：《中药与临床》2014年第6期21-23,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家科技重大专项课题-重大新药创新专项资助项目(2012ZX09103201-014);国家自然科学基金重点基金资助项目(81130066;81227802)

摘　　要：目的:建立得力生注射液中黄芪甲苷的含量测定方法。方法:采用高效液相色谱和蒸发光检测器的方法,Grace Alltima C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水(32∶68,v/v),检测器温度100℃,载气流量为2.7L.min-1,柱温30℃,流速1 m L.min-1。进样量为20μL。结果:黄芪甲苷在1.03μg^26.2μg之间呈现良好的线性关系,r=0.9993,平均回收率为100.1%(RSD=3.7%)。结论:建立的得力生注射液中黄芪甲苷含量测定方法简单快速、准确可靠、重复性好,可有效控制得力生注射液质量。Objective： To establish a method for the determination of Astragaloside A in Delisheng Injection. Method： The chromatographic conditions of HPLC and the parameters of ELSD were optimized to study the methodology. Analysis was performed on Grace Alltima C18 column（4.6 mm×250 mm, 5 μm） with isocratic elution of acetonitrile–water（32∶68） at the fl ow rate of 1.0 m L.min^-1. Drift tube temperature was maintained at 100℃ and the gas fl ow rate was 2.7 L.min^-1. Result： The calibration curve was linear in the range from 1.03 μg to 26.20 μg（r=0.9993）. The recovery of astragaloside A were 100.1%（RSD=3.7%）. Conclusion： This method is rapid and accurate, and can effectively control the quality of Delisheng Injection.

关 键 词：得力生注射液 黄芪甲苷 含量测定 HPLC-ELSD 

分 类 号：R284.1[医药卫生—中药学]