运用生物信息学方法鉴别易混种射干和鸢尾  

Identification of Belamcanda chinensis and Iris tectorum Species Based on Gen Bank Nucleotide Database rbc L Sequence

作　　者：陈莹[1] 

机构地区：[1]福建农林大学园林学院,福建福州350002

出　　处：《佳木斯职业学院学报》2014年第12期173-173,179,共2页Journal of Jiamusi Vocational Institute

基　　金：福建省教育厅科技项目(JB13052)

摘　　要：利用Gen Bank核酸数据库中rbc L序列鉴定观赏药用植物易混品种射干和鸢尾。在Gen Bank核酸数据库下载射干和鸢尾的rbc L序列,NCBI虚拟PCR方法获取共有的对应片段,应用MEGA5.0软件计算种内种间(K2P)遗传距离和构建邻接(NJ)系统聚类树。获得4个射干rbc L序列及7个鸢尾rbc L序列,这些序列在30bp-630bp之间具有对应序列,基于该段序列分析显示射干和鸢尾之间的K2P遗传距离0.01,远大于鸢尾种内遗传距离(0.001)与射干种内遗传距离(0);NJ系统聚类树结果表明能将射干和鸢尾区分开来,分别单独聚为一类。结果表明:所选rbc L序列适用于射干和鸢尾的鉴别,可为射干和鸢尾准确应用提供鉴定依据。To identify Belamcanda chinensis and Iris tectorum Species by using GenBank nucleicacid database rbcL sequence. Based on GenBank Nucleotide Database, rbcL sequences of Belamcanda chinensis and Iris tectorum species were downloaded. K2P Distances were calculated using MEGA 5.0 software and neighbor-ioining （NJ） phylogenetic tree was established. K2P genetic distance between Belamcanda chinensis and Iris tectorum is 0.01, which was far more than intraspecific genetic distance of Belamcanda chinensis （0） and Iris tectorum （0.001）. Results of NJ phylogenetic tree showed that Belamcanda chinensis can be separated from Iris tectorum species and classified into a separate one. rbcL sequence is suitable for the identification of Belamcanda chinensis and Iris tectorum and provides reference for quality and safety identification of Belamcanda chinensis and Iris tectorum.

关 键 词：射干 鸢尾 RBC L序列 DNA条形码 混淆品种 

分 类 号：Q811.4[生物学—生物工程]