溴代香豆素对胃癌细胞的细胞毒活性与致死机制研究  

Cytotoxicity and Death Mechanism of Bromine Coumarin for Gastric Carcinoma Cells

作　　者：郭耀[1] 

机构地区：[1]兰州城市学院化学与环境科学学院,甘肃兰州730090

出　　处：《西北民族大学学报（自然科学版）》2014年第3期40-45,共6页Journal of Northwest Minzu University(Natural Science)

基　　金：2013年甘肃省高等学校第二批科研项目(2013B-073);甘肃省教育科学"十一五"规划2011年度规划课题(GS[2011]GHBG009)项目

摘　　要：为探究合成化合物溴代香豆素的生物活性,将该化合物作用于胃癌细胞进行了体外研究.本研究分别采用磺酰罗丹明染色法和生长曲线法研究了药物的细胞毒活性和癌细胞的致死过程.以彗星电泳技术和瑞士—吉姆萨染色法观察了化合物对细胞核形态变化和核酸损伤效应,最后以琼脂糖凝胶电泳法检测了药物对细胞总DNA的影响.结果显示,溴代香豆素对胃癌细胞具有显著的细胞毒活性,其IC50为21.56μg/m L.药物对细胞的增殖抑制效应和致死效应都具有显著的时间—剂量依赖性.药物作用癌细胞后可引起细胞核形态变化和染色质凝集断裂,并损伤细胞DNA,在电泳中呈梯度降解状态.结果表明,溴代香豆素对胃癌细胞的生长与增殖具有显著的细胞毒活性,并能诱导胃癌细胞发生凋亡.To study bioactivity of Bromine coumarin, gastric carcinoma cells were treated with the drug in vitro. Cytotoxicity and death of cancer cells were studied by the methods of sulfonyl rhodarnine B staining method （SRB） and growth curve, and DNA damage and nuclear morphometry were detected through comet assay and Swiss- Gimsa staining method, and DNA degradation also was determined by Agarose gel electrophoresis. The results showed that Bromine coumarin had a significant cytotoxicity for the cancer cells, and IC50 was 21.56 ？ g/ml. There were significant dose and time dependent manners in proliferation inhibition and lethal effect. The drug led to nuclear morphometry change and fracture of condensed chromatin, and DNA damage. Drug also induced a ladder degradation of DNA in gel electrophoresis. In conclusion, the bromine coumarin has a significant cytotoxity on gastric carcinoma cells, and induced cell apoptosis.

关 键 词：溴代香豆素 胃癌细胞 细胞毒活性 DNA损伤 细胞凋亡 

分 类 号：R285[医药卫生—中药学]