miR-208转染中国仓鼠肺成纤维细胞后炎症基因程序性细胞死亡因子-4表达的改变  

Changes in expression of inflammatory gene PDCD4 after transfection with miR-208 in Chinese hamster lung fibroblasts

作　　者：李文超[1] 劳灿山 李明月[1] 张迎建[1] 朱显明[1] 史冰[1] 燕思雨 王之卉[1] 杨红[2] 

机构地区：[1]东南大学公共卫生学院 环境医学工程教育部重点实验室,江苏南京210009 [2]东南大学公共卫生学院环境医学工程教育部重点实验室,江苏南京210009

出　　处：《环境与健康杂志》2014年第10期889-892,F0003,共5页Journal of Environment and Health

基　　金：国家自然科学基金面上项目(81273046);国家重大科学研究计划项目(2011CB933404);江苏省预防医学科研课题(Y2012039);环境医学工程教育部重点实验室开放课题基金(2011EME009)

摘　　要：目的研究miR-208转染中国仓鼠肺成纤维(CHL)细胞对炎症相关靶基因程序性细胞死亡因子-4(PDCD4)的调控作用。方法运用在线数据库Targetscan.org预测前期纳米二氧化硅经气管一次性灌注大鼠实验中筛选出差异表达上调miR-208可能调控的靶基因,并进行基因本体论(GO)分析,通过文献检索选择与炎症相关的靶基因为PDCD4;分别用miR-208模拟物、模拟物阴性对照转染CHL细胞,并设空白对照组(完全培养基),测定细胞增殖率和miR-208及PDCD4的mRNA表达水平及PDCD4蛋白的表达水平。结果预测miR-208可能调控的靶基因有146个。与空白对照组相比,转染后CHL细胞的增殖率差异无统计学意义(P>0.05),miR-208 mRNA的表达增加(P<0.01),PDCD4 mRNA和蛋白的表达无统计学意义(P>0.05)。结论在CHL细胞中未见miR-208对PDCD4有间接调控作用,是否存在直接调控作用有待进一步研究。Objective To study the role of miR-208 to regulate inflammation related target genes PDCD4 in CHL cells.Methods Online database Targetscan.org and Gene Ontology analysis were used to predict the possible target genes of the miR-208 chosen from the preliminary rats experiments of nano-SiO2 disposable intratracheal instillation. PDCD4 was selected by literature search. The CHL cells were transfected with miR-208 mimics and mi RNA mimics negative control respectively,and blank control group（complete medium）. The proliferation rate of CHL cells,the expression of mi R-208 and PDCD4 m RNA and the protein of PDCD4 were determined. Results miR-208 could regulate 146 target genes. Compared with the blank control group, the proliferation rate of CHL cells remained no change（P〉0.05）, the expression level of miR-208 mRNA was significantly higher（P〈 0.01）,both the expressions of PDCD4 m RNA and protein remained no change（P 〉0.05） after transfection. Conclusion There is no indirect regulation of miR-208 and its predicted target gene PDCD4 in CHL cells.Whether there is a direct regulation of miR-208 to PDCD4 remains further experimental validation.

关 键 词：miR-208 程序性细胞死亡因子-4 中国仓鼠肺成纤维细胞 肺损伤 

分 类 号：R994.6[医药卫生—毒理学]