妊娠期邻苯二甲酸(2-乙基己基)酯暴露对成年后子代雄鼠精子的致突变作用  

Mutagenicity of prenatal DEHP exposure to sperm of sexually matured male offspring rats

作　　者：张钰[1] 刘永哲[1] 高娜[1] 张华婧[1] 黄玉敬 张彩凤[1] 石东星 孙增荣[1] 

机构地区：[1]天津医科大学公共卫生学院,天津300070

出　　处：《环境与健康杂志》2014年第10期909-911,共3页Journal of Environment and Health

基　　金：国家自然科学基金(81273027)

摘　　要：目的探讨妊娠期邻苯二甲酸(2-乙基己基)酯(DEHP)暴露对成年后子代大鼠精子的致突变作用。方法将32只健康SPF级SD孕鼠随机分为4组,分别为对照(玉米油)组和2、10、50 mg/kg DEHP染毒组,每组8只。自GD13至GD19,采用灌胃方式进行,每天1次。每窝随机选取1只初生雄性仔鼠,观察其生殖器的发育情况;于70日龄时,观察精子的畸形情况。结果妊娠期DEHP暴露雄性仔鼠的体重、肛殖距系数(AGI)及主要生殖器(睾丸、附睾、精囊腺)的脏器系数与对照组比较,差异无统计学意义(P>0.05);主要生殖器未见畸形。2 mg/kg及50 mg/kg DEHP染毒组子代雄性大鼠精子的头部畸形率和总畸形率均高于对照组,差异有统计学意义(P<0.05);而各剂量DEHP染毒组子代雄性大鼠的尾部畸形率与对照组比较,差异均无统计学意义(P>0.05)。结论在本实验条件下,妊娠期较低(2 mg/kg)及较高(50 mg/kg)剂量DEHP暴露对子代雄鼠的精子均具有遗传毒性作用。Objective To investigate the mutagenic effects of prenatal DEHP exposure on sperm in adult offspring rats by sperm abnormality test. Methods A total of 32 SPF healthy pregnant SD rats were randomly divided into 0,2,10,50 mg/kg body weight DEHP exposure dose groups,eight rats in each group. The pregnant rats were treated through gavage from gestation day 13（GD13） to GD19 once a day. One male offspring rat per litter was randomly selected after postnatal. At the 70th day after birth（PND70）,the epididymal was picked and sperm smears were made for the examination. Results There was no significant difference in male offspring rats body weight among the groups and index of anogenital distance（AGI）. No abnormality of main reproductive organ was observed,and there was no significant difference in reproductive organ coefficient among the groups.The total abnormality rates and head abnormality rate of sperm of 2,50 mg/kg DEHP exposure groups were significantly higher than that of the control group（P〈0.05）. There was no significant difference among four DEHP dose groups in tail abnormality rate. Conclusion Prenatal exposure to low dose（2 mg/kg） and high dose（50 mg/kg） DEHP may have genetic toxicity to germ cells of the male offspring rats.

关 键 词：邻苯二甲酸(2-乙基己基)酯 精子畸形 遗传毒性 

分 类 号：R994.6[医药卫生—毒理学]