超声波辅助提取香蕉叶多糖工艺的优化  

Optimization of ultrasonic-assisted extracting polysaccharide from banana leaves

作　　者：林士惠[1] 易运红[1] 龙梓 吴功庆[1] 

机构地区：[1]广东药学院医药化工学院,广东中山528458

出　　处：《南方农业学报》2014年第2期295-298,共4页Journal of Southern Agriculture

基　　金：广东药学院大学生创新创业训练项目(1057313023)

摘　　要：【目的】优化超声波辅助提取香蕉叶多糖工艺条件,为香蕉叶的深度开发提供理论参考。【方法】以经脱脂脱单糖后的香蕉叶为材料,在单因素试验基础上,以提取温度、提取时间、超声波功率为影响因素,应用Box-Behnken中心组合法进行3因素3水平试验,以香蕉叶多糖含量为响应值,进行响应面分析,优化提取工艺条件。【结果】香蕉叶多糖含量对提取温度、提取时间及超声波功率的二次多元回归方程为:Y=1.42+0.064A+0.065B+0.00775C-0.057A2-0.057B2+0.003525C2+0.022AB-0.0075AC+0.006BC,由模型得出香蕉叶多糖最佳提取条件为:提取温度66.35℃、提取时间28.73 min、超声波功率150 W。为方便试验,最终确定超声波辅助提取香蕉叶多糖的最佳工艺条件为:提取温度66℃、提取时间29 min、超声波功率150 W,此条件下提取获得的香蕉叶多糖含量1.40%。【结论】超声波辅助提取香蕉叶多糖能够降低提取温度,极大缩短提取时间,是高效的提取方法。【Objective】The ultrasonic-assisted extraction process of polysaccharide from banana leaves was optimized to provide theoretical references for further development of banana leaves. 【Method】Banana leaves without monosaccharide after degrease were used as research materials. On the basis of single factor experiments, a three-factor and three-level Box-Behnken central composite design was utilized for mathematical modeling of polysaccharide yield. Influencing factors included extraction temperature, extraction time and ultrasonic power. With polysaccharide extraction rate as response value, response surface analysis was made to optimize extraction conditions. 【Result】 Polysaccharide content in banana leaves on the coding independent variables were extraction temperature, extraction time and ultrasonic power and the quadratic multinomial regression equation was Y=1.42＋0.064A＋0.065B＋0.00775C-0.057A2-0.057B2＋0.003525C2＋0.022AB-0.0075AC＋0.006BC. The optimum conditions for the extraction of polysaccharide from banana leaves were as follows： extraction temperature of 66.35 ℃, extraction time of 28.73 min and ultrasonic power of 150 W. The polysaccharide yield of the experiment was 1.40% under the conditions of extraction temperature of 66 ℃, extraction time of 29 min and ultrasonic power of 150 W. 【Conclusion】Ultrasonic-assisted extraction of polysaccharide from banana leaves could decrease extraction temperature and greatly reduce the extraction time. So the extraction method was efficient.

关 键 词：香蕉叶 多糖 响应面法 超声波提取 工艺条件优化 

分 类 号：TQ914.1[化学工程]