枸杞果实酸性转化酶基因生物信息学分析  

Bioinformatics Analysis of Acid Invertase Gene of Lycium Bararum Fruit

作　　者：雷建武[1] 门惠芹[1] 李浩霞[2] 尹跃[2] 赵建华[2] 

机构地区：[1]宁夏农林科学院,宁夏银川750002 [2]宁夏农林科学院枸杞工程技术研究中心,宁夏银川750002

出　　处：《宁夏农林科技》2014年第12期17-19,22,共4页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：国家自然科学基金资助(31360191);宁夏回族自治区自然科学基金(NZ13125);宁夏农林科学院先导资金项目(NKYJ-14-07)

摘　　要：借助生物信息学工具对已克隆枸杞果实酸性转化酶蛋白LBSAI的理化性质、亲水性/疏水性、跨膜结构域、二级结构和三级结构等方面进行预测和分析。结果表明：枸杞果实酸性转化酶蛋白为不存在信号肽亲水性蛋白;在氨基酸第37-39位置间产生一个跨膜螺旋结构;该蛋白二级结构主要有无规则卷曲和延伸链构件,并散布于整个蛋白。研究为进一步研究枸杞果实酸性转化酶基因调控蔗糖代谢途径机理提供理论参考。The physicochemical properties, hydrophilicity/hydrophobicity, transmembrane domains, two level structure and three stage structure of the cloned lycium bararum fruit acid invertase were calculated and analyzed by using bioinformatics tools.The results showed that the lycium bararum fruit acid invertase is a hydrophilicity protein without a signal peptide and it produces a transmembrane helix structure in the 37-39 article location of the amino acids. The two level structure of the protein mainly has a random coil and the extended chain component. It provides a theory reference information for further researching the mechanism of the regulation of sucrose metabolism pathway of the lycium bararum fruit acid invertase gene.

关 键 词：枸杞果实 酸性转化酶蛋白 生物信息学分析 

分 类 号：S567.19[农业科学—中草药栽培]