Plackett-Burman试验设计联用星点设计-效应面法优化纤维素酶水解淫羊藿苷为宝藿苷Ⅰ的工艺  

Optimization of Process of Icraiin be Hydrolyzed to BaohuosideⅠby Cellulase Based on Plackett-Burman Design Combined with CCD Response Surface Methodology

作　　者：宋川霞[1] 陈红梅[1] 戴宇[1] 康敏[1] 胡佳[1] 邓赟[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《中药材》2014年第11期2082-2086,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81373961);国家科技部新药创制重大专项(2014ZX09201021-008);四川省科技厅省青年科技创新研究团队专项计划项目(2014TD0007)

摘　　要：目的:运用Plackett-Burman试验设计联用星点设计(Central Composite Design,CCD)-效应面法,考察各影响因素显著性,筛选出用纤维素酶水解淫羊藿苷为宝藿苷Ⅰ的最佳工艺。方法:运用Plackett-Burman试验设计筛选影响酶反应的主要因素,选用CCD-效应面法优化纤维素酶水解淫羊藿苷为宝藿苷Ⅰ的最佳工艺。以底物浓度、缓冲液p H、反应时间为自变量,淫羊藿苷的转化率为因变量,利用星点设计模型进行二项式拟合,利用相应的三维曲面图直观分析纤维素酶水解淫羊藿苷为宝藿苷Ⅰ的最佳工艺,并进行验证试验及预测分析。结果:纤维素酶水解淫羊藿苷为宝藿苷Ⅰ的最佳工艺为:底物浓度为8.23 mg/m L,缓冲液p H为5.12,反应时间为35.34 h。结论:利用Plackett-Burman试验设计联用CCD-效应面法确定了纤维素酶水解淫羊藿苷为宝藿苷Ⅰ的最佳工艺,该方法简便、重现性好、预测性强。Objective： To optimize the process of Icraiin be hydrolyzed to Baohuoside Ⅰ by cellulase by Plackett-Burman design combined with Central Composite Design（ CCD） response surface methodology. Methods： To select the main influencing factors by Plackett-Burman design,using CCD response surface methodology to optimize the process of Icraiin be hydrolyzed to BaohuosideⅠ by cellulase. Taking substrate concentration,the pH of buffer and reaction time as independent variables,with conversion rate of icariin as dependent variable,using regression fitting of completely quadratic response surface between independent variable and dependent variable,the optimum process of Icraiin be hydrolyzed to BaohuosideⅠ by cellulase was intuitively analyzed by 3D surface chart,and taking verification tests and predictive analysis. Results： The best enzymatic hydrolytic process was as following： substrate concentration 8. 23 mg / mL,pH 5. 12 of buffer,reaction time 35. 34 h. Conclusion： The optimum process of Icraiin be hydrolyzed to BaohuosideⅠ by cellulase is determined by Plackett-Burman design combined with CCD response surface methodology. The optimized enzymatic hydrolytic process is simple,convenient,accurate,reproducible and predictable.

关 键 词：淫羊藿苷 宝藿苷Ⅰ 纤维素酶 PLACKETT-BURMAN 星点设计 水解工艺 

分 类 号：R286.1[医药卫生—中药学]