肾康注射液对高血压肾损害大鼠肾脏的影响  

Effect of Shenkang Injection on Kidney Function in Hypertensive Renal Damage Rats

作　　者：张宇[1] 周楠[1] 李婷[1] 王红英[1] 贺建宇[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061

出　　处：《中药材》2014年第12期2248-2254,共7页Journal of Chinese Medicinal Materials

摘　　要：目的:观察肾康注射液对高血压肾损害大鼠血压、代谢、血液生化、肾脏组织病理学等的影响,为临床试验提供理论依据。方法:将75只高血压肾损伤SHR随机分为5组:模型组、苯那普利阳性对照组及肾康注射液低、中、高剂量组,每组15只,以WKY为正常对照组(n=15)。低、中、高剂量组分别给予肾康注射液6.7、13.3、26.6m L/kg,相当于生药2、4、8 g/kg。模型组和正常对照组给予等体积生理盐水,苯那普利组给药剂量8 mg/kg。给药方式为腹腔注射。结果:连续腹腔注射给药3个月,肾康注射液能抑制SHR大鼠血压升高;显著降低SHR尿液中尿微量白蛋白及尿蛋白浓度;降低血肌酐(Scr)和尿素氮(BUN)含量;提高内生肌酐清除率(Ccr)和血清白蛋白(ALB)含量及超氧化物歧化酶(SOD)活性,降低血清中丙二醛(MDA)、醛固酮(Ald)、肿瘤坏死因子(TNF-α)、白介素(IL-6)、转化生长因子(TGF-β1)和C反应蛋白(CRP)的含量;各组大鼠肾脏指数与心脏系数差异均无统计学意义,肾脏病理切片显示给药后肾小球纤维化明显降低,炎细胞减少,肾小管纤维化减轻。结论:肾康注射液具有改善高血压肾损害大鼠肾脏功能、降低血清炎症因子水平、改善氧化应激状态及减轻高血压肾损害程度等作用。Objective： To observe the effect of Shenkang Injection on the blood pressure,metabolism,blood biochemistry and renal pathology in hypertension renal damge rats,then to provide theoretical basis for clinical trials. Methods： 75 spontaneously hypertensive nephropathy rats were randomly divided into five groups with 15 rats in each group： model group（SHR group） rats were intragastrically treated with the vehicle（4 m L/kg normal saline per day） of Shenkang Injection per day; Benazepril group（positive control group,8 mg /kg Benazepril per day）; Shenkang Injection low-dose group（6. 7 m L/kg Shenkang Injection per day）; middle-dose group（13. 3 m L/kg Shenkang Injection per day）; high-dose group（26. 6 m L/kg Shenkang Injection per day）; and WKY rats were normal control group（n= 15）（4 m L/kg normal saline per day）. Results： After 3 months intraperitoneal injection treatment,SHR rats blood pressure were inhibited; the levels of microalbumin（m-ALB）,total protein（U-TP）,serum creatinine（Scr） and urea nitrogen（BUN） were decreased significantly in Shenkang Injection treated groups rats. Shenkang Injection significantly improved the levels of creatinine clearance rate（Ccr）,serum albumin（ALB） and superoxide dismutase（SOD）,decreased the content of methane dicarboxylic aldehyde（MDA）,aldosterone（Ald）,tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,transforming growth factor-β1（TGF-β1） and C-reactive protein（CRP）,and had histologic improvement compared with model group. Conclusion： Shenkang Injection can improve the kidney function,decrease the levels of serum inflammatory factors,improve the oxidative status and reduce the degree of hypertensive renal damage.

关 键 词：肾康注射液 高血压肾损害 血压 肾功能 炎症因子 

分 类 号：R285.5[医药卫生—中药学]