淫羊藿总黄酮对D-半乳糖致H9c2细胞衰老的保护作用研究  

Protective Effect of Total Flavonoids of Epimedium on D-galactose-induced Senescence in H9c2 Cells

作　　者：李靳[1] 张长城[1] 杨莉[1] 王婷[1] 周志勇 袁丁[1] 顿耀艳[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002

出　　处：《中药材》2014年第12期2255-2258,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81100957,81374001,81273895,81373881);自然科学基金创新群体项目(2013CFA014)

摘　　要：目的:研究淫羊藿总黄酮(Total Flavonoids of Epimedium,TFE)对D-半乳糖诱导H9c2细胞衰老模型的影响及其机制。方法:采用不同浓度TFE干预D-半乳糖作用的H9c2细胞,以细胞形态学和β-半乳糖苷酶染色检验细胞衰老情况,并检测超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、细胞内活性氧(ROS)水平以及细胞凋亡情况。结果:各浓度TFE均能显著降低D-半乳糖所致的β-半乳糖苷酶染色阳性的细胞数目、MDA含量及ROS荧光强度,并能升高SOD活性,改善细胞核染色质浓缩及减少凋亡小体。结论:TFE可通过抗氧化和减少细胞早期凋亡来对抗D-半乳糖所致H9c2细胞衰老。Objective： To investigate the protective effect of Total Flavonids of Epimedium（TFE） on D-galactose-induced H9c2 cells senescence and to explore the underlying mechanisms. Methods： D-galactosed（50 mmol/L） were used to induce the aging of H9c2 cells. Different concentrations of TFE（5,25 and 50 μg/m L） were added into the medium before the use of D-galactosed. Cells senescence was identified by β-galactosidase and the changes in cell morphology were observed. The activity of superoxide dismutase（SOD）and the content of malondial dehyde（MDA） were measured. Reactive oxygen species（ROS） was observed by DCFH-DA detection and the apoptosis was tested by Hochest. Results： In the D-gal group,the number of β-galactosidase-positive cells,the content of MDA and the fluorescence intensity of ROS were markedly increased. In the meanwhile,the chromatin was condensated and the apoptotic bodies were seen by using Hochest. Compared with the D-gal group,TFE apparently decreased the quantity of β-galactosidase-positive cells,the content of MDA,and the florescence intensity of ROS,increased the activity of SOD,and enhanced the condition of chromatin. Conclusion： TFE can confront D-galactose-induced senescence in H9c2 cells by increasing the ability of antioxidant and reducing the number of apoptosis.

关 键 词：淫羊藿总黄酮 D-半乳糖 H9C2细胞 衰老 

分 类 号：R285.5[医药卫生—中药学]