三类钠盐阴离子表面活性剂对CHO细胞增殖活性的影响  

Toxic Effect of Proliferation on CHO Cells Induced by Three Kinds of Ionic Surfactants

作　　者：张博文[1] 王晓宁[1] 廖青[1] 

机构地区：[1]北京服装学院材料科学与工程学院,北京100029

出　　处：《北京服装学院学报（自然科学版）》2014年第4期1-5,12,共6页Journal of Beijing Institute of Fashion Technology：Natural Science Edition

基　　金：北京市教委"科技成果转化与产业化--北京市中小学校服安全技术规范及监管体系研究(提升计划项目)(TJSHS201310012002)

摘　　要：探讨三类钠盐阴离子表面活性剂分子结构与其细胞增殖活性之间的关系.运用噻唑蓝比色法检测三类钠盐阴离子表面活性剂对CHO细胞增殖活性的影响,并用半数抑制浓度IC50值表征其毒性大小.结果表明:三类钠盐阴离子表面活性剂的促进作用随其碳链长度的增加而减小;三类钠盐阴离子表面活性剂的抑制作用随其碳链长度的增加而增大;细胞毒性由大到小为烷基硫酸酯钠盐,烷基磺酸钠盐,烷基羧酸钠盐.To explore the three kinds of anionic surfactant sodium salt in the Chinese hamster ovary cells （CHO） proliferation activity of influence. MTT assay method was used to detect the toxicity of 3 kinds of Ionic Surfactants on CHO cells. Then Compared its toxicity in terms of its half inhibitory concentration （ IC50 alue）. It is turned out that the promoting effect of the 3 kinds of anionic surfac- tants was decreasing with the chain length＇ s growth. And their cytotoxicity increased with the growth of compound chain length. The eytotoxieity of alkyl sulfate sodium was the strongest, the cytotoxicity of alkyl sulfonie acid sodium salt was stronger, both stronger than alkyl carboxylic acid salt under the same chain length.

关 键 词：阴离子表面活性剂 细胞毒性 噻唑蓝比色法 IC50 

分 类 号：Q26[生物学—细胞生物学]