基于IFA技术检测微线体蛋白2在艾美耳属球虫的空间分布  

ANALYSIS ON THE LOCATION OF MICRONEME 2 PROTEIN IN SPOROZOITES OF EIMERIA SPP. BY INDIRECT IMMUNOFLUORESCENCE ASSAY

作　　者：田秀玲[1] 汤新明[1] 秦梅[1] 索静霞 刘贤勇 索勋[1] 

机构地区：[1]中国农业大学动物医学院,北京100193

出　　处：《寄生虫与医学昆虫学报》2014年第4期240-243,共4页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金重点项目（No.31330076）

摘　　要：微线体蛋白2（Microneme protein2，Mic2）是艾美耳球虫入侵宿主细胞时分泌的主要功能蛋白之一。本研究中，以抗柔嫩艾美耳球虫微线体蛋白2（EtMic2）单克隆抗体为检测抗体，利用间接免疫荧光实验（IFA）检测了Mic2在柔嫩艾美耳球虫、和缓艾美耳球虫、斯氏艾美耳球虫和无残艾美耳球虫等不同种艾美耳属球虫的空间分布。结果显示，Mic2在不同种艾美耳属球虫中均定位于子孢子的顶部（微线体部位），但表达强度存在差异。这提示Mic2可作为研究艾美耳属球虫相关蛋白空间分布的“参照物”。Microneme protein 2 （Mic 2） is the structural protein of microneme of Eimeria spp. The secretion of Mic 2 is very important during the process of invading the host cells. In this study, we used a monoclonal antibody specific to E. tenella Mic 2 （EtMic2） to detect the Mic 2 location in several Eimeria spp. , i. e. , E. teneUa, E. mitis, E. stiedai and E. irresidua, by indirect immunofluorescence assay （IFA）. The Mic 2 was located in the anterior region of the sporozoites among all Eimeria spp. , which indicated that Mic 2 is highly conserved among Eimeria spp. and could be used as a marker protein when studying the location of Eimeria spp. proteins.

关 键 词：艾美耳属球虫 单克隆抗体 微线体蛋白2 空间分布 间接免疫荧光实验 

分 类 号：S858.315.9[农业科学—临床兽医学]