和缓艾美耳球虫激发宿主免疫应答的初步研究  

STUDY OF THE ADAPTIVE IMMUNE RESPONSE STIMULATED BY EIMERIA MITIS INFECTION IN CHICKEN

作　　者：汤新明[1] 秦梅[1] 索静霞 田秀玲[1] 刘贤勇 索勋[1] 

机构地区：[1]中国农业大学动物医学院,北京100193

出　　处：《寄生虫与医学昆虫学报》2014年第4期253-257,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金重点项目（31330076）

摘　　要：和缓艾美耳球虫Eimeriamitis是7种鸡球虫中致病力弱的虫种，对于其免疫原性的认识至今仍有争论。本研究以和缓艾美耳球虫涿州株为研究对象，以初次免疫及二次免疫后鸡的卵囊排出量为标准评估了其免疫原性。同时利用ELISA检测了免疫后鸡群血清中球虫特异性IgY抗体的水平，并通过ELISPOT技术分析了二免后鸡群外周血单核淋巴细胞（PBMC）中分泌IFN-γ的特异性淋巴细胞的数量。结果显示，二次免疫后，鸡的卵囊排出量显著低于初次免疫，免疫组的血清特异性IgY抗体水平较低，而分泌IFN-γ的特异性淋巴细胞的数量较高。这些结果表明和缓艾美耳球虫涿州株具备良好免疫原性，且其激发宿主产生的保护性免疫以细胞免疫应答为主，有作为疫苗株的潜力。Eimeria mitis with low pathogenicity is easily ignored compared with other Eimeria spp. in chicken and the mechanism of difference immunogenicity between different strains is unclear. In this study, E. mitis （Zhuozhou Strain） was used to analysis its immunogenicity by the oocysts output post each immunization. Moreover, We analyzed the humoral immune response and cellular immune response elicited by E. mitis infection via the serum [gY antibody titer detected by ELISA and IFN-~/ secretion cell population post stimulated with E. mitis in peripheral blood mononuclear cell （PBMC） by ELISPOT, respectively. Our results show that the oocyst output was relatively low and the IgY titer was still very low after the second immunization. However, high concentration of IFN-γwas detected in PBMC after stimulated with E. mitis post the second immunization 2 weeks. Our results indicated that strong cellular immune responses elicited by E. mitis could be contributed to its good immunogenicity and thus protect chicken from homologous infection.

关 键 词：和缓艾美耳球虫 免疫原性 体液免疫应答 细胞免疫应答 

分 类 号：S942.5[农业科学—水产养殖]