艾美耳球虫子孢子从宿主细胞中逸出机制的初步研究  

PRELIMINARY STUDY ON THE MECHANISMS OF RAPID EGRESS OF EIMERIA TENELLA SPOROZOITES FROM HOST CELLS

作　　者：闫鑫磊 计永胜[1] 田秀玲[1] 索静霞 刘贤勇 索勋[1] 

机构地区：[1]中国农业大学动物医学院,北京100193

出　　处：《寄生虫与医学昆虫学报》2014年第4期263-267,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金项目（No.31172309）

摘　　要：艾美耳球虫是一类重要的肠道病原，其裂殖生殖阶段的虫体逸出过程是造成畜禽肠道破坏的主要原因之一，但此逸出过程的机制仍鲜有报道。本研究以乙醇作为诱导剂研究柔嫩艾美耳球虫M2e株子孢子从宿主细胞中逸出的机制。结果显示，乙醇可诱导子孢子从MDBK细胞中逸出，此逸出过程依赖于虫体的运动能力；同时，乙醇可激发子孢子逸出相关的微线体蛋白2（Mic2）的分泌释放。进一步实验证实，螯合虫体内部钙离子明显阻断了子孢子逸出及Mic2蛋白的释放。本研究初步证实了与柔嫩艾美耳球虫逸出相关的蛋白和离子，为深入解析球虫致病的分子机制、研发新型抗球虫药物提供了新的研究方向。Apicomplexan parasites infect nearly all vertebrate hosts even including humans and cause some severe diseases such as malaria, toxoplasmosis, cryptosporidiosis which are responsible for substantial economic losses. Like most intracellular pathogens, egress from host cells is a vital step of the apicomplexan parasites, which attracted attentions of many research groups. Unfortunately, as an important genus of Phylum Apicomplexa, little information of egress is known on Eimeria species which leads large amount of losses to poultry industry annually. In this report we used ethanol to induce egress of E. tenella M2e transgenic strain （EtM2e） sporozoites which express yellow fluorescent protein （YFP）. Results showed that ethanol could induce egress of EtM2e sporozoites from infected Madin Derby Bovine Kidney （MDBK） cells, and this process was depended on the mobility of the parasites. We also found that ethanol could also stimulate microneme protein discharge. Furthermore, both the course of egress and the secretion of microneme were controlled by the flux of intracellular Ca2＋ of the parasite. Taken together, our results preliminarily explained the mechanism of egress of eirnerian parasites, which provided clues to the further study.

关 键 词：柔嫩艾美耳球虫 子孢子 乙醇 逸出 

分 类 号：Q255[生物学—细胞生物学]