L-精氨酸对实验性脑型疟疾的免疫调控机制研究  

Immune Regulatory Mechanisms of L-Arginine in Experimental Cerebral Malaria

作　　者：杨志蕃[1] 庞维[2] 杜云婷 曹雅明[2] 

机构地区：[1]沈阳军区总医院肾内科,辽宁沈阳110016 [2]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001

出　　处：《微生物学杂志》2014年第6期65-69,共5页Journal of Microbiology

基　　金：高等学校博士学科点专项科研基金项目(20112104110018)

摘　　要：在疟疾流行区,L-精氨酸(L-Arg)被认为是一种对疟疾患者安全有效,能逆转内皮细胞功能紊乱的药物。实验主要研究L-Arg对实验性脑型疟疾的作用特点及其免疫调节作用。结果显示,与生理盐水对照组相比,在伯氏疟原虫(P.b ANKA)感染后给予L-Arg,C57BL/6小鼠的原虫血症水平降低,但存活时间却缩短。LArg处理后,脾脏中CD4+T-bet+IFN-γ+Th1细胞百分率显著性增加,同时脾细胞培养上清中IFN-γ、TNF-α以及NO水平也显著性提高。然而,L-Arg处理后未见Treg细胞的百分率及IL-10的显著性变化。由此提示,L-Arg通过增加小鼠的Th1应答,在脑型疟疾发生时加速小鼠的死亡。因此,应重新评估L-Arg对脑疟的防治效果。In malaria endemic areas,L-arginine（ L-Arg）,was considered to be a safe and effective medicine that can reverse endothelial cell function disorder for malarian patients. The effect feature and immune modulation of L-Arg on experimental cerebral malaria were mainly studied. The results showed that,as compared with control group using physiological saline,in C57 BL /6 mice given L-Arg after P. berghei（ Pb ANKA） infection the level of parasitemia was lowered,but the survival time shortened. After treated with L-Arg,the percentage of CD4＋T-bet＋IFN-γ＋Th1 cell in spleen was significantly increased,while the levels of IFN-γ,TNF-α and NO in the culture supernatants of spleen cells were also significantly improved. However,percentage of Treg cells and the level of IL-10 had no significant changes after treated with L-Arg. Thus prompted that L-Arg increased Th1 response in mice,and hastened the death of mice when cerebral malaria occurs. Therefore,the control effect of L-Arg on preventing cerebral malaria should be revalued.

关 键 词：疟疾 L-精氨酸 TH1免疫应答 调节性T细胞 

分 类 号：Q93-3[生物学—微生物学]