肺炎支原体感染鼠P1特异抗原的动态检测  

Dynamic Detection of P1 Specific Antigen in Mycoplasma pneumoniae Infected Mice

作　　者：赵芝娜[1,2] 张晗[3] 何梦博[1] 陈博[1] 杨颖[1] 司文[1] 王桂珍[1] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,辽宁沈阳110001 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [3]中国医科大学附属二院儿科,辽宁沈阳110004

出　　处：《微生物学杂志》2014年第6期84-88,共5页Journal of Microbiology

基　　金：辽宁省社会科学发展基金项目(辽科发2012-46)

摘　　要：通过实验动物模型探讨肺炎支原体感染动物肺泡灌洗液中特异抗原检出率的动态变化,为肺炎支原体感染的临床诊断提供理论依据。小鼠经鼻自然感染肺炎支原体,分别采集感染后不同时间点小鼠的支气管灌洗液,应用量子点标记肺炎支原体P1蛋白抗体,直接免疫荧光法检测感染鼠肺泡灌洗液中肺炎支原体P1特异抗原,同时通过PCR检测肺组织肺炎支原体DNA及肺组织病理切片观察肺部炎性变化确定小鼠感染。结果显示,感染鼠肺炎支原体特异抗原在感染后第3天检出阳性率为75%,第7天达高峰为83%,之后随病程延长,抗原检测的阳性率逐渐下降,在感染后第14、21天检出阳性率分别为58%和25%。肺炎支原体特异抗原在感染早期检出率高。应用量子点标记肺炎支原体P1蛋白抗体,直接免疫荧光法检测肺炎支原体特异抗原可应用于肺炎支原体感染的早期诊断。The dynamic variance of Mycoplasma pneumoniae infected animals＇ specific antigen in pulmonary alveolar lavage fluid（ PALF） was investigated through M. pneumoniae（ Mp） infected animal models,in order to provide a foundation for clinical diagnosis of Mp infection. BALB / c mice were infected by intranasal inoculation of Mp. Bronchial lavage fluid samples of infected mice in different times were respectively collected after infection to detect the specific antigen of Mp P1 protein by quantum dots label antibody. And tested the Mp specific antigen in infected mice PALF with direct immunofluorescence method; meanwhile detected Mp DNA in pulmonary tissue with PCR and pathological section of pulmonary tissue to observe the inflammatory variance in order to ascertain the mice were infected. The results showed that the positive detection rate at 3 day after the infection,the Mp specific antigen of infected mice was75% and reached to a peak of 83% at 7 day after the infection. It can be concluded that in the early infection Mp specific antigen the detection rate was high. With the application of quantum dots labeling Mp P1 protein antibody,and direct immunofluorescence method to detect Mp specific antigen can be applied to early diagnosis of Mp infection.

关 键 词：肺炎支原体 特异抗原 动态检测 

分 类 号：Q939.91[生物学—微生物学] R446.62[医药卫生—诊断学]