聚丁二酸丁二醇酯降解菌株的筛选及其降解性能研究  

Screening for a Poly-Butylene-Glycol-Succinate(PBS) Degrading Strain and Its Degradation Capability

作　　者：白俊岩[1] 毛海龙[1] 王战勇[1] 

机构地区：[1]辽宁石油化工大学化学化工与环境学部,辽宁抚顺113001

出　　处：《微生物学杂志》2014年第6期98-101,共4页Journal of Microbiology

基　　金：国家自然科学基金项目(31100099);辽宁石油化工大学大学生创新创业训练计划项目(2012008)

摘　　要：从石油污染土壤中筛选到1株具有聚丁二酸丁二醇酯降解能力的菌株PBS1302,经菌体形态特征、菌落培养特征、生理生化鉴定和16S r DNA基因序列分析,初步鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。该菌株在培养温度37℃,培养基起始p H 6.8的条件下经6 d的培养,对聚丁二酸丁二醇酯薄膜的降解率可达36.9%。经电子显微镜观察,与降解前相比,聚丁二酸丁二醇酯薄膜表面变得粗糙,出现明显的蚀刻痕迹。A strains PBS1302 which can degrade poly-butylene-glycol-succinate（ PBS） isolated from petroleum polluted soil was initially identified as Pseudomonas aeruginosa by its morphology,colony and cultivation features,physiological and biochemical characterization,and 16 S r DNA gene sequence analysis. The degradation rate reached by36. 9% when cultured 6 days under the conditions at 37 ℃ and initial p H at 6. 8. The PBS films surfaces became rough and appeared apparent etching notch observed by SEM as compared with the one before the degradation.

关 键 词：聚丁二酸丁二醇酯 降解 铜绿假单胞菌 

分 类 号：Q939.9[生物学—微生物学]