DCPTA对低温下玉米叶片生理生化特征的影响  

Effects of DCPTA on the Physiological and Biochemical Characteristics in Maize Seedling Leaves Under Low Temperature Stress

作　　者：朱恒光 顾万荣[1,2] 王泳超[1] 姜籽竹 谢腾龙[1] 李晶[1,2] 魏湜[1,2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]农业部东北地区作物栽培科学观测实验站,黑龙江哈尔滨150030

出　　处：《核农学报》2015年第3期549-556,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31201164);中国博士后基金(2012M511434);黑龙江省博士后基金(LBH-Z12036)

摘　　要：本文探究了DCPTA[2-(3,4-二氯苯氧基)-乙基-二乙胺]提高玉米抗冷性的调控机理以及叶片喷施的最适浓度,从而减缓低温对玉米苗期的迫害。以丰禾1(低温敏感型)、久龙5(抗冷型)2个玉米品种为试材,在14℃/5℃(昼/夜)低温条件下,研究不同浓度的DCPTA对玉米幼苗3种抗氧化酶活性(SOD、CAT和POD)、可溶性蛋白和可溶性糖含量、膜透性和丙二醛(MDA)含量以及DPPH(1,1-二苯基-2-三硝基苯肼)活性氧清除效率的影响。结果表明,在低温胁迫条件下,不同浓度DCPTA处理提高了玉米幼苗叶片的3种抗氧化酶(SOD、CAT和POD)活性,叶片MDA含量降低,显著提高了玉米幼苗叶片可溶性糖、可溶性蛋白含量和DPPH活性氧清除效率。在低温胁迫下,50 mg·L-1的DCPTA增加了渗透调节物质的含量及提高了抗氧化酶的活性和DPPH活性氧清除效率,从而减少了活性氧(ROS)及膜脂过氧化产物MDA在叶片中的积累,增强了2种不同抗冷型玉米幼苗耐低温胁迫的能力。50 mg·L-1DCPTA显著提高了丰禾1和久龙5的抗低温能力,与久龙5相比,丰禾1的相对电导率和MDA含量的增加幅度平均低出11.3%;抗氧化酶活性的增加幅度平均高出140.0%;DPPH活性氧清除效率的增加幅度高出38.1%;可溶性蛋白和可溶性糖含量的增加幅度高出73.2%,说明DCPTA施用后对低温敏感型品种玉米效果更佳。本研究为探究DCPTA提高玉米苗期抗冷性的生理机制提供理论基础。This article explores the DCPTA（ 2-（ 3,4-dichlorobenzene oxygen radicals）-ethyl-diethylamine） regulation mechanism on improving the cold resistance of maize and the optimal spraying concentration,thus to slow down the persecution of low temperature on maize seedling stage. Two maize varieties,Fenghe1（ low temperature sensitive） and Jiulong5（ cold resistance） as experimental materials,under the low temperature condition of 14℃ /5℃（ day / night）.The effects of different concentrations DCPTA on the activity of three kinds of antioxidant enzymes（ CAT、SOD and POD）,cell membrane permeability and MDA content,DPPH（ 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（ 2,4,6-trinitrophenyl） hydrazyl） clearance rate of reactive oxygen species,soluble protein content and soluble sugar content. Under low temperature stress,the results showed that different concentrations DCPTA can improve SOD、POD and CAT activity in maize; reduce the content of MDA; significantly improve soluble protein content,soluble sugar content in maize and DPPH clearance rate of Reactive oxygen species. Under low temperature stress,appropriate concentrations DCPTA can improve the activity of antioxidant enzymes, the contents of antioxidants and osmotic regulation substances,thereby reducing the accumulation of reactive oxygen species（ ROS） and membrane lipid peroxidation products MDA in leaf,enhancing the resistance of two different types of seedlings against low temperature stress. The most suitable concentration is 50 mg·L- 1,and it is more effective to improve the cold resistance range of cold sensitive maize to low temperature stress. Providing theoretical evidence for the physiological mechanisms of exploring the effect of increasing cold resistance of maize seedlings by DCPTA.

关 键 词：玉米 低温胁迫 生理机制 调控效应 

分 类 号：S513[农业科学—作物学]