Phosphomonoesterase Activities,Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years  

作　　者：LI Liang LIANG Xin-Qiang LI Hua JI Yuan-Jing LIU Jin YE Yu-Shi TIAN Guang-Ming CHEN Ying-Xu LUO Yong-Ming 

机构地区：[1]Institute of Environmental Science and Technology,College of Environmental and Resources Sciences,Zhejiang University [2]Zhejiang Academy of Agricultural Sciences [3]Key Laboratory for Water Pollution Control and Environmental Safety [4]Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences

出　　处：《Pedosphere》2015年第2期294-306,共13页土壤圈（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21077088,41271314and 51008107)

摘　　要：Soil phosphomonoesterase plays a critical role in controlling phosphorus(P) cycling for crop nutrition,especially in P-deficient soils.A 6-year field experiment was conducted to evaluate soil phosphomonoesterase activities,kinetics and thermodynamics during rice growth stages after consistent swine manure application,to understand the impacts of swine manure amendment rates on soil chemical and enzymatic properties,and to investigate the correlations between soil enzymatic and chemical variables.The experiment was set out in a randomized complete block design with three replicates and five treatments including three swine manure rates(26,39,and 52 kg P ha^(-1),representing low,middle,and high application rates,respectively) and two controls(no-fertilizer and superphosphate at 26 kg P ha^(-1)).The results indicated that the grain yield and soil chemical properties were significantly improved with the application of P-based swine manure from 0 to 39 kg P ha^(-1);however,the differences between the 39(M_(39)) and 52 kg P ha^(-1) treatments(M_(52)) were not significant.The enzymatic property analysis indicated that acid phosphomonoesterase was the predominant phosphomonoesterase in the tested soil.The M_(39) and M_(52) treatments had relatively high initial velocity(V_0),maximal velocity(V_(max)),and activation grade(lgN_a) but low Michaelis constant(K_m),temperature coefficient(Q_(10)),activation energy(E_a),and activation enthalpy(ΔH),implying that the M_(39) and M_(52) treatments could stimulate the enzyme-catalyzed reactions more easily than all other treatments.The correlation analysis showed that the distribution of soil phosphomonoesterase activities mainly followed the distributions of total C and total N.Based on these results,39 kg P ha^(-1) could be recommended as the most appropriate rate of swine manure amendment.Soil phosphomonoesterase plays a critical role in controlling phosphorus（P） cycling for crop nutrition,especially in P-deficient soils.A 6-year field experiment was conducted to evaluate soil phosphomonoesterase activities,kinetics and thermodynamics during rice growth stages after consistent swine manure application,to understand the impacts of swine manure amendment rates on soil chemical and enzymatic properties,and to investigate the correlations between soil enzymatic and chemical variables.The experiment was set out in a randomized complete block design with three replicates and five treatments including three swine manure rates（26,39,and 52 kg P ha^-1,representing low,middle,and high application rates,respectively） and two controls（no-fertilizer and superphosphate at 26 kg P ha^-1）.The results indicated that the grain yield and soil chemical properties were significantly improved with the application of P-based swine manure from 0 to 39 kg P ha^-1;however,the differences between the 39（M39） and 52 kg P ha^-1 treatments（M52） were not significant.The enzymatic property analysis indicated that acid phosphomonoesterase was the predominant phosphomonoesterase in the tested soil.The （M39） and （M52） treatments had relatively high initial velocity（V0）,maximal velocity（Vmax）,and activation grade（lgNa） but low Michaelis constant（K_m）,temperature coefficient（Q10）,activation energy（Ea）,and activation enthalpy（ΔH）,implying that the M39 and M52 treatments could stimulate the enzyme-catalyzed reactions more easily than all other treatments.The correlation analysis showed that the distribution of soil phosphomonoesterase activities mainly followed the distributions of total C and total N.Based on these results,39 kg P ha^-1 could be recommended as the most appropriate rate of swine manure amendment.

关 键 词：activation energy activation enthalpy enzyme-catalyzed reaction maximal velocity Michaelis constant 

分 类 号：S154.2[农业科学—土壤学]