华细辛气相色谱指纹图谱及药材含量测定研究  

GC fingerprint of Asarum sieboldii Miq.

作　　者：于游[1] 马海英[1] 牛思佳[1] 陈霞[1] 赵春杰[2] 

机构地区：[1]中国医科大学附属第四医院药学部,沈阳110032 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中南药学》2015年第2期116-118,共3页Central South Pharmacy

摘　　要：目的建立华细辛挥发油的气相色谱（GC）指纹图谱并采用内标法对α-蒎烯、莰烯和甲基丁香酚3种成分进行定量分析。方法采用水蒸气蒸馏法提取华细辛中的挥发油,利用毛细管气相色谱对其进行分析,采用DB-1石英毛细管柱（25 m×0.32 mm,0.25μm）,氢火焰离子化检测器,程序升温,流速为1m L·min^-1,进样量为1μL,分流比为10：1,内标物为丁香酚。结果获得了较为理想的华细辛GC指纹图谱,确定了19个共有峰,建立了华细辛指纹图谱的共有模式,10批样品相似度均〉0.95;α-蒎烯、莰烯和甲基丁香酚分别在0.024 0-0.768 0 mg·m L^-1、0.005 0-0.160 0 mg·m L^-1及0.066 0-2.112 0 mg·m L^-1线性关系良好,平均回收率分别为92.0%、95.6%和97.8%,RSD分别为3.7%、1.7%和2.4%。结论该方法建立的华细辛指纹图谱和3种成分的定量测定方法,可为华细辛药材的质量评价提供依据。Objective To establish a GC fingerprint method for Asarum sieboldii Miq. and to determine the content of α-pinene, camphene and methyl isoeugenol simultaneously. Methods The sample was extracted by the oil extractor and determined with capillary column DB-17（30 m×0.25 mm×0.25 μm） by GC coupled with flame ionization detector. Programmed temperature was performed at 1.0 m L·min^- 1 with injection volume was 1 μL. Split injection was conducted with split ratio of 10 ： 1. The internal standard was isoeugenol. Results A preferable method for GC fingerprint of Asarum sieboldii Miq. with 19 common peaks achieved. The common pattern was developed. The similarities of 10 batches of the samples were all over 0.95. α-pinene, camphene and methyl isoeugenol were linear at 0.024 0- 0.768 0 mg·m L^- 1, 0.005 0- 0.160 0 mg·m L^- 1 and 0.066 0- 2.112 0 mg·m L^- 1, respectively. The average recoveries were 92.0%, 95.6% and 97.8%（RSD 3.7%, 1.7% and 2.4%）. Conclusions This GC fingerprint and the content determination method of α-pinene, camphene and methyl isoeugenol can be used for the quality control of Asarum sieboldii Miq..

关 键 词：华细辛 气相色谱指纹图谱 含量测定 Α-蒎烯 莰烯 甲基丁香酚 

分 类 号：R917[医药卫生—药物分析学] R282[医药卫生—药学]