树莓‘沙尼’组培技术  

Tissue Culture for Orus of Raspberry(Rubus ideaus L.) Cultivar

作　　者：霍朗宁[1] 张东升[2] 贾忠奎[1] 

机构地区：[1]省部共建森林培育与保护教育部重点实验室(北京林业大学),北京100083 [2]国家林业局

出　　处：《东北林业大学学报》2015年第3期6-9,共4页Journal of Northeast Forestry University

基　　金：新疆维吾尔自治区科技计划项目(201231108);北京林业大学大学生创新创业训练项目(120104)

摘　　要：应用树莓（Rubus ideaus L.）的优良品种‘沙尼’（‘Shawnee’）进行组织培养技术的探索和研究,以期为树莓的大量繁育和推广提供技术支撑。结果表明：最佳外植体的选择为带芽茎段,最佳的取材时间为5月份,萌发率可达100%。最佳灭菌体系的流程为75%酒精浸泡30 s后无菌水冲洗3~4次,然后再以2%Na Cl O灭菌20 min后无菌水冲洗3次,污染率达到0%。‘沙尼’的最佳初代培养基为MS＋6-BA 1.0 mg·L-1＋IAA 0.5 mg·L-1;最佳继代培养基为MS＋6-BA 0.5 mg·L-1＋NAA 0.05 mg·L-1,增殖系数为3.49;最佳生根培养基为1/2MS＋IBA 1.0 mg·L-1,生根率为100%,植株可平均生出2 cm的健壮根系10条。In order to breed and popularize the raspberry efficiently,we focused on the Shawnee,a fine-cultivar of raspberry（Rubus ideaus L.）,and studied the technology system of tissue culture.The stem section with a bud is an optimum explant while spring in May is an optimum time with the germination rate of 100%.After soaking with 75% alcohol for 30 s and flushing with sterile water for 3-4 times,2% Na Cl O to sterilize 20 min before flushing by sterile water for 3 times is an optimum program of sterilization without the contamination.MS＋6-BA 1.0 mg·L-1＋IAA 0.5 mg·L-1is an optimum early generation culture medium,MS＋6-BA 0.5 mg·L-1＋NAA 0.05 mg·L-1is an optimum subculture generation culture medium（4.55 proliferation ratio）,and 1 / 2MS＋IBA 1.0 mg·L-1is an optimum strike root culture medium（100% root productive ratio,two individual of strong roots and mean length of 10 cm）.

关 键 词：树莓 '沙尼’ 组织培养 

分 类 号：S663.2[农业科学—果树学] Q943.1[农业科学—园艺学]