多年生黑麦草花粉活力和柱头可授性  

Pollen Viability and Stigma Receptivity of Lolium perenne

作　　者：翟飞飞[1] 巨关升[1] 李伟[1] 刘俊祥[1] 韩蕾[1] 孙振元[1] 

机构地区：[1]中国林业科学研究院林业研究所,北京100091

出　　处：《东北林业大学学报》2015年第3期75-78,共4页Journal of Northeast Forestry University

基　　金：国家"863"计划项目(2011AA100209)

摘　　要：以多年生黑麦草(Lolium perenne)品种‘Pinnacle’为材料,用TTC染色法和花粉管体外萌发法测定花粉在4种贮藏条件下(4℃常态、4℃干燥、-20℃常态和-20℃干燥)的活力变化;采用联苯胺—过氧化氢法测定开花前和开花后不同时段的柱头可授性。结果表明:多年生黑麦草花粉不耐贮藏,相比较而言,4℃干燥有利于其保存,7 d后花粉活力可维持在5%左右;而柱头可授性在开花前1 d至开花后5 h较强,该时间内授粉有利于受精作用,可提高结实率。With Lolium perenne commercial variety‘Pinnacle＇,we used TTC staining method and pollen tube germination method in vitro to measure pollen viability under four storage conditions of 4 ℃ and room humidity,4 ℃ and dry condition,-20 ℃and room humidity,and-20 ℃ and dry condition.And we used benzidine-hydrogen peroxide method to study stigma.The pollen of Lolium perenne was not resistant to storage,and 4 ℃ with dry condition was beneficial to its conserve relatively.Pollen viability maintained about 5% after 7 d.Stigma receptivity was strong between 1 d before blooming and 5 h after blooming,and this period was beneficial to fertilization and enhancing fruiting rate of flowers.

关 键 词：多年生黑麦草 花粉活力 柱头活性 TTC染色 花粉管体外萌发 

分 类 号：S512.5[农业科学—作物学] Q944.42[生物学—植物学]