梅花鹿鹿茸组织Anxa-1基因cDNA克隆及表达  

Cloning and Expression Analysis of Anxa-1 Gene c DNA from Sika Deer Antler Tissue

作　　者：曲昊淼 丁玲[1] 赵姬臣 夏彦玲[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2015年第3期99-103,共5页Journal of Northeast Forestry University

基　　金：中国博士后基金(20110491124)

摘　　要：从梅花鹿(Cervus nippon)鹿茸间充质中成功克隆出包括膜联蛋白A1(Anxa-1)基因全部编码区的c DNA序列,并结合生物学信息方法及实时荧光定量技术对该基因的氨基酸序列及不同生长时期的表达情况进行了分析。结果表明:Anxa-1基因编码346个氨基酸。经生物学信息分析,该基因编码蛋白为稳定蛋白,不具备信号肽,相对分子质量为38 830.4,理论等电点为6.17,一级结构中亮氨酸占最大比例(10.4%)。同源序列比对及系统进化树分析表明,梅花鹿Anxa-1氨基酸序列与藏羚羊(Pantholops hodgsonii)、绵羊(Ovis aries)、山羊(Capra hircus)和水牛(Bubalus bubalis)相似性较高。实时荧光定量RT-PCR分析表明,Anxa-1基因在不同生长时期鹿茸顶端间充质表达存在差异,生长前期的表达量高于中期与后期。We cloned the c DNA sequence including all the coding region of the Anxa-1 gene from sika deer antler mesenchymal for the first time,and used bioinformatics method and real-time RT-PCR to analyze the amino acid sequence and the expression at different phases.The Anxa-1 gene encoded a peptide of 346 amino acid residues of which the relative molecular weight was 38 830.4.By analyzing biological information,the gene had an isoelectric point of 6.17,and did not contain a signal peptide; Leucine accounted for the largest proportion in the primary structure was 10.4%.Homologous sequence alignment and phylogenetic tree analysis indicated that the Anxa-1 mature protein of sika deer was highly similarity with Pantholops hodgsonii,Ovis aries,Capra hircus and Bubalus bubalis.By real-time RT-PCR,the gene expression at different phases had specificity,and the expression of the Anxa-1 gene was higher at its earlier stage than that at its intermediate and advanced stage.

关 键 词：膜联蛋白A1 鹿茸 c DNA 克隆 实时荧光定量RT-PCR 

分 类 号：S825.2[农业科学—畜牧学]