3种地格达类蒙药材中獐牙菜苦苷和当药黄素的初步比较研究  

Preliminary comparative study of swertiamarin and swertisin on three kinds of Digeda-species Mongolian medicinal materials

作　　者：吕颖 张海涛 王艳芳[2] 朱虹[2] 龙平[2] 王振旺[2] 张娜[2] 张春红[2] 

机构地区：[1]赤峰市药品检验所,内蒙古赤峰024000 [2]包头医学院,内蒙古包头014060

出　　处：《中国中药杂志》2015年第5期804-806,共3页China Journal of Chinese Materia Medica

基　　金：国家"十二五"科技支撑计划项目(2012BAI28B02);国家自然科学基金项目(81060372;81160504);内蒙古自治区教育厅项目(NJZY13239)

摘　　要：肋柱花、扁蕾和尖叶假龙胆分别为龙胆科肋柱花属、扁蕾属和假龙胆属植物,三者均可作为地格达类蒙药广泛用于肝胆等疾病的治疗,临床效果显著。该文采用高效液相色谱法对内蒙古地区3种蒙药材中獐牙菜苦苷和当药黄素进行初步比较研究,旨在为临床安全用药提供科学依据。文中所采用色谱柱为Phenomenex C18（4.6 mm×250 mm,5μm）,流动相为甲醇（A）和水（B）梯度洗脱,流速1.5 m L·min-1,柱温25℃,检测波长237 nm。结果显示3种蒙药中均含有獐牙菜苦苷和当药黄素,其含量与植物种类和产地关系密切。不同产地肋柱花中獐牙菜苦苷质量分数为1.73%~2.72%,当药黄素质量分数为0.43%~0.96%;阿拉善左旗产扁蕾中獐牙菜苦苷的质量分数为0.38%;其他产地扁蕾及尖叶假龙胆中獐牙菜苦苷和当药黄素均为定性检出。因此,从肋柱花等3种蒙药中獐牙菜苦苷、当药黄素的初步测定结果分析,三者化学成分因种类和产地不同而差异较大,临床用药需按疾病种类不同仔细斟酌,不宜盲目混用。Lomatogonium rotatum （L.） Fries,Gentianopsis barbata （Froel） Ma,and Gentianella acuta （Michx.） Hulten,the three kinds of Digeda-species Mongolian medicinal materials belonging to the family Gentianaceae ,had been widely used for the treatment of liver diseases. To analyze comparatively the content of swertiamarin and swertisin among these three kinds of Digeda-species Mongolian medicinal materials. HPLC method was applied for qualitative and quantitative analysis of swertiamarin and swertisin. The Phenomenex C18 （4.6 mm ×250 ram,5 μm） was used,chromatographic methanol and water as mobile phase,the flow rate was 1.5 mL·min-1 with UV detected at 237 nm, column oven temperature was 25℃. Results showed that the contents of swertiamarin and swertisin were close- ly related the different species and producing areas. The content range of swertiamarin in L. rotatum from different habitats was 1.73%-2. 72% ,0.43%-0.96% for the swertisin content; the content of swertiamarin in G. barbata from Alxa Left Banner was 0. 38% , and the content of swertiamarin and swertisin in G. barbata from the others habitats and G. Acuta from different habitats were all detected qualitatively. The contents of swertiamarin and swertisin among these medicinal plants showed a significant difference due to the different species and producing areas. As a consequence,these medicinal plants should not be put together for clinical applications.

关 键 词：肋柱花 扁蕾 尖叶假龙胆 獐牙菜苦苷 当药黄素 

分 类 号：R29[医药卫生—民族医学]