红腺忍冬绿原酸生物合成新基因克隆及其生物信息学分析  

Gene cloning and bioinformatics analysis of new gene for chlorogenic acid biosynthesis of Lonicera hypoglauca

作　　者：余淑琳 黄璐琦[1,2] 袁媛[2] 齐琳洁 刘大会[3] 

机构地区：[1]安徽中医药大学,安徽合肥230038 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [3]云南省农业科学院药用植物研究所,云南昆明650000

出　　处：《中国中药杂志》2015年第5期863-867,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373959);国家杰出青年科学基金项目(81325023)

摘　　要：为挖掘红腺忍冬绿原酸生物合成的关键酶基因,应用生物信息学方法从红腺忍冬基因组数据库中筛选出4个新基因LHPAL4,LHHCT1,LHHCT2,LHHCT3,并预测其编码蛋白的结构和功能。序列分析结果表明,红腺忍冬LHPAL4与LHPAL1关系密切;LHHCT1,LHHCT3,LHHQT2关系密切,LHHCT2单独聚为一类。通过Real-time PCR检测4个新基因在红腺忍冬不同组织中的转录情况,发现LHPAL4,LHHCT1,LHHCT3在败花中表达量最高,LHHCT2在叶中的表达量最高,为揭示不同部位活性成分含量差异机制提供依据,也为活性成分的合成生物学提供元件。To obtain the key genes for chlorogenic acid biosynthesis of Lonicera hypoglauca, four new genes ware obtained from the our dataset of L. hypoglaaca. Xnd we also predicted the structure and function of LHPAL4, LHHCT1, LHHCT2 and LHHCT3 proteins. The phylogenetic tree showed that LHPAL4 was closely related with LHPAL1, LHHCT1 was closely related with LHHCT3, LHHCT2 clustered into a single group. By Real-time PCR to detect the gene expressed level in different organs of L. hypoglauca, we found that the transcripted level of LHPAIA, LHHCT1 and LHHCT3 was the highest in defeat flowers, and the transcripted level of LHHCT2 was the highest in leaves. These result provided a basis to further analysis the mechanism of active ingredients in different organs, as well as the element for in vitro biosynthesis of active ingredients.

关 键 词：红腺忍冬 活性成分 基因家族 变异分析 

分 类 号：S567.79[农业科学—中草药栽培]