基于等温滴定量热法的注射用益气复脉(冻干)临床联合用药相容性研究  

Compatibility research of Yiqi Fumai injection with clinical combination based on isothermal titration calorimetry

作　　者：刘红宇[1] 马丽娜[2] 张萍[2] 冯雪[2] 肖小河[2] 刘绍贵[1] 张雅铭[2] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]解放军302医院全军中药研究所,北京100039

出　　处：《中国中药杂志》2015年第5期889-893,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目(81330090);国家自然科学基金项目(8117542)

摘　　要：为了能快速、灵敏、较全面反映中西药注射剂临床联合用药相互作用,该文采用等温滴定量热法(isothermal titration calorimetry,ITC),以注射用益气复脉(冻干)为模式药,考察其分别与盐酸肾上腺素和5%葡萄糖注射液的相容性,以热力学参数吉布斯自由能(ΔG)、焓变(ΔH)、熵变(ΔS)判断溶合反应类型,通过对比不同注射剂混合前后化学指纹图谱特征信息对结果进行佐证。结果表明,注射用益气复脉(冻干)与盐酸肾上腺素滴定过程中测得|ΔH|>T|ΔS|,为焓驱动反应,且释放热量较大,提示两者之间的相互作用以化学反应为主,内在物质发生改变。注射用益气复脉(冻干)与5%葡萄糖溶液溶合时|ΔH|<T|ΔS|,为熵驱动反应,且反应活性谱显示放热较少,提示两者之间的相互作用以物理反应为主,活性成分仅被溶解稀释,未发生质变。ITC法可用于注射用益气复脉(冻干)临床联合用药相互作用表征的研究,亦可用作其他中药注射剂临床联合用药相容性评价。To reveal the characterization of interaction between Chinese and western medicinal injections, isothermal titration calo- rimetry （ITC） was applied to evaluating the interaction of Yiqi Fumai injection （ YQFM, as mode drug） with epinephrine hydrochloride injection （YS） and 5% glucose injection （5% GS）. The diversification of Gibbs free energy（AG）, enthalpy（AH）, and entropy （AS） were determined to judge the reaction types of colliquefaction procedures of different injections. Meanwhile, the fingerprints of YQFM before and after combined with the various injections were compared to validate the results. This work demonstrated that during the titration procedure of YQFM and YS, IAHI 〉 TIASI, that was to say the reaction was enthalpy-driving. And the reactive profile indicated that a great deal of heat gave out during the procedure. Obviously, chemical reactions happened and the internal component changed. On the other side, the reaction of YQFM combined with 5% GS was entropy-driving, because IAHI 〈 TIASI. The reactive profile showed there was only a little heat released. So non-chemical reactions happened and the major ingredients did not change. ITC could be applied to the evaluation on compatibility of other kinds of Chinese and western medicinal injection combination.

关 键 词：等温滴定量热法 注射用益气复脉(冻干) 盐酸肾上腺素注射液 葡萄糖注射液 联合用药 

分 类 号：R969.2[医药卫生—药理学]