紫草素对IL-17诱导角质形成细胞增殖及趋化因子表达的影响  

Effect of shikonin on proliferation of keratinocytes induced by interleukin-17 and expression of chemokines

作　　者：解欣然[1] 张蕾[1] 刘欣[1] 林燕[1] 张璐[1] 李萍[1] 

机构地区：[1]首都医科大学附属北京中医医院北京市中医研究所,北京100010

出　　处：《中国中药杂志》2015年第5期946-949,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81072810);北京市中医管理局青年基金(QN2012-10)

摘　　要：目的:观察紫草素(shikonin)对IL-17诱导人角质形成细胞增殖及其分泌趋化因子的影响,探讨紫草素治疗银屑病的作用机制。方法:采用IL-17A(200μg·L-1)刺激体外培养的Ha Ca T细胞,同时加入不同浓度紫草素(2,1mg·L-1)共同作用24 h。CCK-8法测定细胞增殖;ELISA法测定细胞分泌炎症因子IL-23;Real-time PCR法测定细胞内趋化因子CXCL1,CXCL2和CCL20以及β-防御素4(β-defensin4,DEFB4)的表达。结果:紫草素2,1 mg·L-1能显著抑制IL-17A诱导的Ha Ca T细胞增殖,具有统计学意义(P<0.01);紫草素各浓度组均能减少IL-23分泌,并对细胞内CXCL1,CXCL2,CCL20和DEFB4的表达有一定的抑制作用。结论:紫草素能抑制IL-17A诱导的Ha Ca T细胞增殖和相关细胞因子的分泌,并可以通过抑制趋化因子募集白细胞,从而达到对银屑病的治疗作用。Objective： To observe the effect of shikonin on the proliferation of human keratinocytes induced by IL-17 and secretion of chemokines, in order to discuss the mechanism of Shikonin in the treatment of psoriasis. Method： In vitro cultured HaCaT cells were stimulated by IL-17A （200μg·L-1 ） and mixed with different concentrations （2, 1 mg ·L-1 ） of shikonin for 24 hours. The cell proliferation was detected by CCK-8 assay. Cell secretion inflammatory factor intedeukin-23 （ IL-23 ） was detected by ELISA. The expressions of intracellular ehemokines CXCL1, CXCL2, CCL20 and fl-defensin 4 （ DEFB4 ） were detected by Real-time PCR. Re- sult： Shikonin （2,1 mg·L-1） could distinctly inhibit HaCaT cell proliferation induced by IL-17A, with statistical difference （P 〈 0.01 ）. Each shikonin group showed decreases in the secretion of IL-23 and inhibition in expressions of intracellular CXCL1, CXCL2, CCL20 and DEFB4. Conclusion： Shikonin could inhibit HaCaT cells proliferation induced by IL-17 and secretion of relevant cytokines and recruit leukocytes by inhibiting ehemokines, so as to show the effect in treating psoriasis.

关 键 词：紫草素 IL-17 HACAT细胞 趋化因子 银屑病 

分 类 号：R275.9[医药卫生—中西医结合]