FKN、PI3K及NF-κB对人外周血单个核细胞IL-6表达的的影响及缬沙坦的干预作用  

Effect of Chemotatic Factor FKN,PI3K and NF-κB on IL-6 Expression in Peripheral Blood Monocytes and the Effect of Valsartan Intervention

作　　者：于霏[1] 金元哲[1] 雷明明[1] 张学颖[1] 张晓春[1] 刘君[1] 

机构地区：[1]中国医科大学附属第四医院心血管内科,沈阳110032

出　　处：《中国医科大学学报》2015年第3期214-216,共3页Journal of China Medical University

基　　金：辽宁省科技厅资助项目(2011404013-6)

摘　　要：目的探讨不规则趋化因子Fractalkine(FKN)、磷脂酰肌醇-3激酶(PI3K)、核因子κB(NF-κB)对外周血单个核细胞(PBMC)IL-6表达的影响及缬沙坦的干预作用,研究FKN影响IL-6表达的信号转导机制。方法利用密度梯度离心法分离健康人外周血单个核细胞,将提取的PBMC分为7组:空白对照组、FKN组、LY294002组(PI3K抑制剂)、PDTC组(NF-κB抑制剂)、FKN+缬沙坦组、FKN+LY294002组、FKN+PDTC组,后二组在FKN诱导PBMC细胞前分别由LY294002、PDTC预处理。各组PBMC分别在12 h和24 h后,用酶联免疫吸附实验(ELISA)法检测各组细胞培养液中的IL-6表达。结果培养12 h后,与对照组比较,FKN组IL-6明显降低(P<0.05),LY294002组和PDTC组无明显差异(P>0.05);与FKN组比较,FKN+缬沙坦组IL-6明显降低(P<0.05),FKN+LY294002组IL-6明显升高(P<0.05)、FKN+PDTC组IL-6明显降低(P<0.05)。培养24 h后,各组IL-6表达无明显差异(P>0.05)。结论 FKN对人外周血单个核细胞IL-6的表达具有双向调节作用,通过PI3K途径抑制IL-6的表达,而通过NF-κB途径促进IL-6的表达,总体上FKN可以抑制IL-6的表达。缬沙坦可增加FKN抑制IL-6表达的作用。Objective To explore effect of irregular chemotatic factor fractalkine（FKN）,phosphatidyl inositol 3 kinase and nuclear factor-κB（NF-κB）on interleukin-6（IL-6）expression in peripheral blood monocytes and the effect of valsartan intervention,so as to research the signal conduc-tive mechanism of FKN impacting on IL-6. Methods Peripheral blood monocytes were isolated from fresh blood of healthy volunteers by the densi-ty gradient centrifugation. The extractive peripheral blood monocytes were divided into seven groups：the control group,the FKN group,the LY294002 group（PI3K inhibitors）,the PDTC group（NF-κB inhibitors）,the FKN＋valsartan group,the FKN＋LY294002 group,and the FKN＋PDTC group,the latter two were pretreated by LY294002 and PDTC respectively before FKN inducing PBMC cells. The IL-6 expression in cell me-dium was measured in each group by ELISA at 12 hours and 24 hours after PBMC treatment. Results After 12 hours of culture,compared with the control group,the expression of IL-6 in the FKN group was decreased（P〈0.05）,while LY294002 and PDTC groups had no significant difference （P〉0.05）. Compared with the FKN group,the expression of IL-6 was decreased in the FKN＋valsartan group（P〈0.05）,increased in the FKN＋LY294002 group（P〈0.05）,and was decreased in the FKN＋PDTC group（P〈0.05）. After 24 hours of culture,the IL-6 expression in each group had no significant difference（P〉0.05）. Conclusion FKN can adjust the expression of peripheral blood PBMC IL-6 in a two-way pattern, inhibiting the expression of IL-6 by PI3K pathway and promoting the expression of IL-6 by NF-κB pathway,overall,FKN can inhibit the expression of IL-6. Valsartan can increase FKN to inhibit the expression of IL-6.

关 键 词：不规则趋化因子 磷脂酰肌醇-3激酶 核因子ΚB IL-6 缬沙坦 

分 类 号：R541.4[医药卫生—心血管疾病]