UPLC法测定新生脉散液中紫丁香苷的含量  

Content Determination of Syringin in New Shengmaisan Liquid by UPLC

作　　者：刘建海[1] 崔维利[2] 陈涛[2] 孟萌[2] 

机构地区：[1]天津中医药大学研究生院,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《中国药房》2015年第9期1236-1238,共3页China Pharmacy

基　　金：国家科技重大专项课题(No.2010ZX09102-202)

摘　　要：目的：建立测定新生脉散液中紫丁香苷含量的方法。方法：采用超高效液相色谱（UPLC）法。色谱柱为ACQUITY UPLC BEN C18,流动相为乙腈-0.4%磷酸水溶液（5∶95,V/V）,流速为0.2 ml/min,检测波长为265 nm,柱温为30℃,进样量为1μl。结果：紫丁香苷的进样量在0.019 7~0.197 0μg范围内与峰面积呈良好的线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD均〈2%;平均加样回收率为99.01%,RSD=1.27%（n=6）。结论：该方法结果准确、重复性好,可作为新生脉散液中紫丁香苷的含量测定方法。OBJECTIVE：To establish a method for content determination of syringin in New Shengmaisan liquid. METHODS：UPLC method was adopted. The determination was performed on ACQUITY UPLC BEN C18 column with mobile phase consisted of acetonitrile-0.4% phosphoric acid（5∶95,V/V）at the flow rate of 0.2 ml/min. The detection wavelength was 265 nm,the column temperature was 30 ℃ and the sampling volume was 1 μl. RESULTS：There was a good linear relationship between the amount of syringin and peak area in the range of 0.019 7-0.197 0 μg（r=0.999 9）;the RSDs of precision,stability and repeatability tests was less than 2%;the average recovery was 99.01%（RSD=1.27%,n=6）. CONCLUSIONS：The method is accurate and reproducible. It can be used for content determination of syringin in New Shengmaisan liquid.

关 键 词：新生脉散液 超高效液相色谱法 紫丁香苷 含量测定 

分 类 号：R927.2[医药卫生—药学]