双波长HPLC法测定酸性龙胆合剂中龙胆苦苷和橙皮苷的含量  

Content Determination of Gentiopicrin and Hesperidin in Acid Gentian Mixture by Dual-wavelength HPLC

作　　者：王文月[1,2] 刘志宏[1] 黄爱文[1] 宋洪涛[1] 

机构地区：[1]南京军区福州总医院药学科,福州350025 [2]福建医科大学药学院,福州350108

出　　处：《中国药房》2015年第9期1241-1243,共3页China Pharmacy

基　　金：军队医疗机构制剂标准提高科研专项课题(No.13ZJ01)

摘　　要：目的：建立双波长高效液相色谱（HPLC）法同时测定酸性龙胆合剂中龙胆苦苷和橙皮苷的含量。方法：采用HPLC法。色谱柱为Agilent ZORBAX SB-C18,流动相为甲醇-0.1%磷酸水溶液（梯度洗脱）,检测波长为270 nm（龙胆苦苷）、283 nm（橙皮苷）,柱温为30℃,进样量为10μl。结果：龙胆苦苷和橙皮苷的质量浓度分别在14.4~100.8μg/ml（r=0.999 7）、8.0~104μg/ml（r=0.999 7）范围内与峰面积呈良好线性关系;精密度、稳定性、重复性试验的RSD均〈2%;平均加样回收率分别为99.92%、102.55%,RSD分别为0.41%、1.84%（n均为9）。结论：该方法简便、快速、灵敏,适用于酸性龙胆合剂中龙胆苦苷和橙皮苷的含量测定。OBJECTIVE：To establish the dual-wavelength HPLC method for content determination of gentiopicrin and hesperidin in Acid gentian mixture. METHODS：HPLC was conducted. The analysis was performed on Agilent ZORBAX SB-C18 column with the mobile phase of methanol-0.1% phosphoric acid solution（gradient elution）;the detection wavelength was 270 nm（gentiopicroside）and 283 nm（hesperidin）;the column temperature was 30 ℃ and the volume was 10 μl. RESULTS：The linear range of gentiopicroside was 14.4-100.8 μg/ml（r=0.999 7）and that of hesperidin was 8.0-104 μg/ml（r=0.999 7）;the RSDs of precision,stability,repeatability tests was all less than 2%;average recoveries were 99.92%（RSD=0.41%,n=9） and 102.55%（RSD=1.84%,n=9）,respectively. CONCLUSIONS：The method is simple,accurate and sensitive and can be used for content determination of gentiopicroside and hesperidin in Acid gentian mixture.

关 键 词：酸性龙胆合剂 龙胆苦苷 橙皮苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]