新型石墨烯-壳聚糖/二茂铁衍生物/细胞色素c修饰电极的制备及其用于亚硝酸钠的检测研究  

Fabrication of New GH-CS / Fc-NH_2/Cytc Modified Electrode and Its Application in Detection of Nitrite

作　　者：黄娜[1] 刘美玲[1] 邓建辉[1] 王林萍[1] 张友玉[1] 姚守拙[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析教育部重点实验室,长沙410081

出　　处：《分析化学》2015年第3期325-332,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21305042;21275051;21375037);湖南省自然科学基金(Nos.14JJ4030;13JJ2020)资助;湖南大学化学生物传感与计量国家重点实验室开放课题(No.2013013);湖南省高校科技创新团队资助(湘教通2012[318号]~~

摘　　要：具有共轭结构的分子导线是构筑分子电子器件的重要组成单元,而含有二茂铁单元的该类化合物具有良好的电化学性质,被广泛研究。基于此,本实验通过Sonogashira交叉偶联等反应合成了一种新型二茂铁乙炔衍生物（Fc-NH2）,并经红外（IR）、核磁共振（1H NMR和13C NMR）、质谱（MS）和循环伏安（CV）等方法表征。利用Fc-NH2与石墨烯-壳聚糖（GH-CS）之间的相互作用制备了GH-CS/Fc-NH2复合物,并成功用于细胞色素c（Cytc）的固定,得到GH-CS/Fc-NH2/Cytc修饰电极。研究表明,GH-CS/Fc-NH2/Cytc/GCE在＂0.2 V附近出现一对峰,对应于Cytc的可逆氧化还原峰。此电极对Na NO2有良好的电催化作用,在1×10＂7-1.5×10＂4mol/L范围内,Na NO2浓度与氧化峰电流呈良好的线性关系,检测限低至4×10＂8mol/L。此修饰电极不但可以实现细胞色素c的直接电化学,也可以用于定量检测Na NO2。Highly conjugated linear molecular wires are one of the basic elements for constructing molecular electronic devices. Among these conjugated compounds,ferrocene-terminated compounds as well as their derivatives have been widely studied because of their ideal electrochemical activity. Based on these,a new ferrocene-terminated phenylethynyaniline（ Fc-NH2） was synthesized by Sonogashira cross coupling reaction.The structure of the target compound was identified by infrared（ IR）,nuclear magnetic resonance（ NMR）,mass spectra（ MS） and cyclic voltammetry（ CV）. Combined the graphene-chitosan（ GH-CS） and Fc-NH2,a new composite of GH-CS / Fc-NH2 was prepared and then used for immobilization of Cytc to prepare the GH-CS / Fc-NH2/ Cytc / GCE. It was found from the CV result that a pair of peaks near ＂0. 2 V appeared which can be attributed to Cytc. The GH-CS / Fc-NH2/ Cytc modified GCE showed good catalysis towards Na NO2,and good linear relationship was found in the range of 1×10＂ 7-1. 5 ×10 ＂4mol / L,and the determination limit was bellow 4 ×10＂ 8mol / L. Therefore,cytochrome c（ Cytc） can be immobilized on the GH-CS / Fc-NH2 and the direct electron transfer can be realized between the electrode and solution and shows good prospect for detection of Na NO2.

关 键 词：二茂铁乙炔衍生物 细胞色素C 石墨烯 亚硝酸盐 电催化 

分 类 号：O657.1[理学—分析化学]