AC4原料药含量的HPLC测定方法研究  

Establishment of HPLC Method for the Determination of Active Pharmaceutical Ingredients of AC4

作　　者：林析 薛飞群[1] 王春梅[1] 张丽芳[1] 费陈忠[1] 张晓晓[1] 赵娟[1] 肖遂[1] 王霄旸[1] 张可煜[1] 王米[1] 郑文丽[1] 

机构地区：[1]中国农业科学院上海兽医研究所,农业部动物寄生虫病学重点开放实验室,中国农业科学院兽药安全评价与兽药残留研究重点开放实验室,上海200241

出　　处：《中国家禽》2015年第5期14-17,共4页China Poultry

基　　金：国家高新技术研究发展计划(863计划)(2011AA10A214);公益性行业(农业)科研专项(201303038-1)

摘　　要：为建立测定AC4原料药含量的高效液相色谱（HPLC）测定方法,色谱柱：Diamosil C18色谱柱（4.6 mm×250 mm,5μm）,流动相为甲醇-0.2%磷酸水溶液,65∶35（v/v）,流速为1.0 m L/min,检测波长251 nm。结果显示,AC4浓度在20~200μg/m L范围与峰面积呈现良好的线性关系,回归方程A=43743ρ＋26 016,R2=0.9999（n=5）,平均回收率100.3%,RSD为0.54%,样品至少在8 h内稳定。表明该法快速简捷、准确度高,适用于AC4原料药含量测定。To establish an HPLC method for the determination of active pharaceutical ingredients of AC4,a Diamosil C18column（4.6 mm×250 mm,5 μm）was used. The mobile phase was methonal-0.2% phosphoricacid solution,65∶35（v/v）.The flow rate was 1.0 m L/min and the detection wavelength was 251 nm. The results showed that the line arrange of the method was 20 to 200 μg/m L and the regression equation was A=43743ρ＋26 016,R2=0.9999（n=5）. The average recovery of the assay was 100.3% and the RSD was 0.54%. The sample solution was stable within 8 h. The method was convenient,sentitive,accurate,and reliable for the determination of active pharaceutical ingredients of AC4.

关 键 词：AC4 测定 高效液相色谱法 

分 类 号：S859.79[农业科学—临床兽医学]