金匮肾气丸对阿尔茨海默病大鼠模型海马神经元β-淀粉样蛋白和神经元核抗原表达的作用  

Effects of Jin Kui Shen Qi Wan on Expression of Beta-amyloid and Neu N in Hippocampal Neurons of Alzheimer's Disease Model Rats

作　　者：方兰[1] 张渊[1] 吴英[1] 

机构地区：[1]浙江省海宁市人民医院,314400

出　　处：《心脑血管病防治》2015年第1期24-26,共3页CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT

摘　　要：目的探讨金匮肾气丸对阿尔茨海默病(AD)模型大鼠海马神经元β-淀粉样蛋白(Beta-amyloid)和神经元核抗原(Neu N)表达的影响。方法清洁级健康SD大鼠24只,分成三组:对照组,AD模型组,中药组。采用在单侧海马注射Aβ25-35建立AD大鼠模型,SABC免疫组化方法检测各组大鼠海马神经元中Beta-amyloid和Neu N的表达。结果 1AD模型组与对照组比较,大鼠海马Beta-amyloid阳性细胞数增多(P<0.01),Neu N阳性细胞数减少(P<0.01);2中药组与AD模型组比较,大鼠海马Beta-amyloid阳性细胞减少(P<0.01),Neu N阳性细胞数增加(P<0.01)。结论金匮肾气丸能抑制Beta-amyloid阳性细胞表达,促进Neu N阳性细胞的表达,从而起到防治AD的作用。Objective To evaluate the impact of jin kui shen qi wan on expression of Beta-amyloid and Neu N in hippocampal neurons of Alzheimer＇s disease model rats. Methods 24 clean healthy SD rats were randomly divided into three groups： control group,the AD model group,TCM group. Adopt unilateral hippocampal injection of Aβ25-35 to establish AD model SABC immunohistochemical method to detect the expression of Beta-amyloid and Neu N in hippocampal neurons. Results ①AD model group compared with the control group rats hippocampus Beta-amyloid-positive cells increased（ P〈0. 01） Neu N positive cells decreased（ P〈0. 01）; ② TCM group compared with the AD model group,Beta-amyloid-positive cells in rat hippocampus（ P〈0. 01） reduction in Neu N positive cells increased（ P〈0. 01）. Conclusions Jin kui shen qi wan,prevention and treatment of AD,its role and inhibition of Beta-amyloid-positive cells expressed the party has to promote the expression of Neu N positive cells.

关 键 词：阿尔茨海默病 海马 金匮肾气丸 Β-淀粉样蛋白 神经元核抗原 

分 类 号：R285.5[医药卫生—中药学]