甘蔗脱毒健康种苗中单糖转运蛋白基因差异表达分析  

The Differential Expression of Monosaccharide Transporter Genes in Disease-free Sugarcane Plants

作　　者：王俊刚[1] 赵婷婷[1] 杨本鹏[1] 蔡文伟[1] 张树珍[1] 

机构地区：[1]中国热带农业科学院甘蔗研究中心中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《生物技术通报》2015年第3期115-120,共6页Biotechnology Bulletin

基　　金："863"计划(2013AA102604-1);海南省自然科学基金项目(313078);海南省重大科技项(ZDZX2013023-1)

摘　　要：旨在探讨脱毒处理对单糖转运蛋白表达量的影响,分析了3种甘蔗单糖转运蛋白基因SGT1、SGT2和PST2a在健康种苗和常规种苗不同生长时期的表达量差异。结果显示,在苗期和分蘖期甘蔗脱毒健康种苗叶片中SGT1、SGT2和PST2a表达量高于未脱毒种苗;在拔节期和成熟期甘蔗脱毒健康种苗未成熟茎节中SGT1、SGT2和PST2a表达量也高于未脱毒种苗;而在成熟茎节中表达量没有差异。苗期和分蘖期甘蔗叶片以及未成熟茎节中细胞生长分裂旺盛,需要利用大量单糖以提供能源和生物合成的前体物质。结果表明,该3种单糖转运蛋白基因表达量上升可能提供更多单糖用于细胞代谢,促进脱毒健康种苗的快速生长及最终生物量的积累。The sugarcane yield can be increased by planting disease-free sugarcane seedlings. However, the physiological and molecular mechanisms of this yield increasing are still not clear now. Monosaccharide transporters are one type of key regulatory proteins involved in carbon accumulation and partitioning processes, and play an important role in plants defense process. The differential expression of three monosaccharide transporter genes（SGT1, SGT2 and PST2a）in disease-free and untreated sugarcane plants was studied to clarify the potential function of these genes on sugarcane yields. The expression levels of SGT1, SGT2 and PST2 a in leaves of disease-free plants at seedling and tillering stages were obviously higher than untreated plants. Furthermore, the expression levels of these genes were increased in immature internodes of disease-free plants at elongation and mature stages, but there were no difference in mature internodes. The sugarcane leaves（at seedling and tillering stages）and the immature internodes（at elongation and mature stages）grow rapidly and demand for more monosaccharide to provide energy and the precursor of biosynthesis. The up-regulations of these monosaccharide transporter genes might accelerate the growth and biomass accumulation of disease-free sugarcane plants by increasing the transporting and uptake of monosaccharide.

关 键 词：甘蔗 健康种苗 单糖转运蛋白基因 基因表达 产量 

分 类 号：S566.1[农业科学—作物学]