半滑舌鳎性逆转的遗传特性研究  

Studies on Genetic Features of Sex Reversal in Cynoglossus semilaevis

作　　者：宋超[1,2] 蒋丽[2] 王景伟[2,3] 李晓芳 李耕[5] 张晓慧[6] 王书[2] 刘哲[1] 李恒德[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]中国水产科学研究院生物技术研究中心,北京100141 [3]福建农林大学动物科学学院,福州350002 [4]中捷罗非鱼养殖有限公司,黄骅061108 [5]中国水产科学研究院营口增殖试验站,营口115000 [6]中国水产科学研究院,资源与环境研究中心,北京100141

出　　处：《生物技术通报》2015年第3期207-212,共6页Biotechnology Bulletin

基　　金：中央公益级基本科研业务费项目(2013A0401,2013A0506)

摘　　要：半滑舌鳎雌雄个体生长差异悬殊,由于性逆转而造成的其群体中雌性比例过低大大制约了养殖效率。性逆转是鱼类及两栖类生物性别决定事件中有趣的生物学问题,其发生的遗传机制鲜有研究。在该研究中,分别利用雄鱼和伪雄鱼组建10个半同胞家系,对这10个半同胞家系中的子代雌雄比进行研究,结果发现,2个伪雄鱼的家系,其后代个体中遗传雌性鱼全部逆转为生理雄鱼;在另外8个雄鱼家系中其性逆转比呈连续分布,表现为典型的数量性状特征;半滑舌鳎性逆转的遗传力较低,仅为0.058。以上结果表明,伪雄鱼作为父本的遗传可能为完全的父本效应遗传,性逆转由于其较低的遗传力不适合于做家系选育而适合于做家系内的选育或结合分子标记的遗传评估,以提高雌性比的遗传进展,半滑舌鳎逆转比的数量遗传特征说明其性别决定是多基因作用的结果。The remarkable individual size differences of half-smooth tongue（Cynoglossus semilavi.）between female and male fishes exist. However, the lower ratio of female in cultured populations arise from sex reversal of the females leads to lower production efficiency. In sex-determination of some fishes and amphibians, sex reversal is interesting biology question and its molecular genetic mechanisms are rarely explored. In this study, 10 half-sib families are set up by utilizing two types of male parents ：genetically males and pseudo males which are genetically females. The results showed that the females were all reversed into physiologically male fishes in two families with pseudo male parent. In the other 8 families with normal male parent, the ratio of sex reversal in individual populations presents continuous distribution, which fits for features of QTLs（Quantitative Trait Loci）typically;the heredity of sex reversal is lower, only 0.058. All of these results showed that, the pseudo males as parents, which demonstrate full paternal-effects, the female progenies were all reversed into pseudo males;interfamily selection for improving the genetic advances of sex ratio or genetic evaluation of sex reversal by using genetic markers are advantageous over intrafamily selection for lower heredity of sex-reversal;the continuous distribution of ratio of sex reversal implies the sex-determination for halfsmooth tongue is depended on the interactions among multiple QTLs.

关 键 词：半滑舌鳎 性别决定 性逆转 

分 类 号：S917.4[农业科学—水产科学]