脑创伤后海马区生存素蛋白促进神经再生的研究  

The study of neurogenesis contributed by the expression of survivin in hippocampus after traumatic brain injury

作　　者：张琳[1] 颜荣[1] 刘晓智[1] 赵玉军[1] 陈镭[1] 

机构地区：[1]天津市第五中心医院神经外科,300450

出　　处：《中华实验外科杂志》2015年第3期542-545,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81471175）;天津市滨海新区塘沽科技兴区项目（2013KJXQ03）

摘　　要：目的 观察脑创伤后抗凋亡因子生存素（Survivin）的表达变化及其与内源性神经再生的相关性,探讨Survivin蛋白在促进创伤后内源性神经再生中的作用及其机制.方法 健康成年雄性C57BL/6小鼠80只,随机分为假手术组和创伤组（TBI组）.在创伤（或假手术）后的12 h,1、2、5、7、14 d（n=6）取打击（或假手术）侧的海马,进行Western blot分析.进一步用免疫荧光双染的方法观察Survivin在不同类型细胞的表达（n=4）.结果 Survivin蛋白在创伤后1d表达开始增高（42 003.15,P＜0.05）,2d时到达高峰（90 403.34,P＜0.01）,持续至第7天开始下降.小鼠打击侧海马齿状回颗粒细胞下层的Survivin＋、溴脱氧尿嘧啶核苷（BrdU＋）和双皮质素（DCX＋）细胞均明显增加;绝大多数的Survivin＋细胞呈现BrdU＋,一部分Survivin＋细胞呈现DCX＋.结论 脑创伤后Survivin基因被激活,蛋白表达量明显增高;Survivin蛋白在受伤侧海马的神经干细胞和未成熟神经元中呈现高表达,与神经源性细胞的增殖有相关性.Objective To investigate the expression of survivin after traumatic brain injury （TBI） ; and the relationship between the up-regulation of survivin and endogenous neurogenesis.Further to demonstrate the role of increased survivin to promote the adult neurogenesis,and detect the possible mechanism in this process.Finally to find the approach that can promote restoration of nervous function after TBI.Methods Adult male C57BL/6 mice,total n =80,were randomly divided into sham-operation group and TBI group.Mice in TBI and sham-operation group were sacrificed at 12 hours,1,2,5,7 and 14 days post TBI and sham-operation （n =6 for each point）,and hippocampus tissues from the ipsilateral hemispheres were carried out for Western blotting.Double-label immunofluorescence staining was adopted to detect survivin expression in difference kinds of cells in the hippocampus （n =4）.Results The up-regulation of survivin protein starting at day 1 （42 003.15,P 〈0.05） and lasting to day 7,peaking at day 2 （90 403.34,P 〈 0.01）.The number of survivin ＋,BrdU ＋ and doublecortin （DCX ＋） cells in subgranular zone （SGZ） of ipsilateral hippocampus was significantly increased after TBI by immunofluorescence staining.The results also revealed that the majority of survivin ＋ cells were BrdU ＋ cells,and a part of survivin ＋ cells were DCX ＋ cells in SGZ of dentate gyrus （DG） in the hippocampus.Conclusion Survivin gene was activated follow brain injury.The expression of survivin protein were increased in the hippocampus after TBI.Increased survivin protein is expressed in neural stem cells and immature neurons in ipsilateral hippocampus after TBI,which correlate to the proliferation of neurogenic cell in SGZ of hippocampus.

关 键 词：生存素 脑创伤 海马 成年神经细胞再生 

分 类 号：R651.15[医药卫生—外科学]