钙蛋白酶Ⅰ通过糖原合成酶激酶3β对脊髓损伤后炎症纤维化的调节作用  

Effects of transforming Calpain Ⅰ and glycogen synthase kinase 3β on chronic fibrosis following spinal cord injury

作　　者：刘涛[1] 曹富江[1] 徐云强[1] 冯世庆[1] 

机构地区：[1]天津医科大学总医院骨科,300052

出　　处：《中华实验外科杂志》2015年第3期591-592,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金重点项目（81330042）;国家自然科学青年基金资助项目（81101362、81401784）;国家科技部对俄合作专项项目（2014DFR31210）

摘　　要：目的 观察钙蛋白酶Ⅰ （Calpain Ⅰ）通过糖原合成酶激酶3β（GSK3β）对脊髓损伤后炎症纤维化的调节作用.方法 将20只SD大鼠随机分为对照组、模型组.通过反转录-聚合酶链反应（RT-PCR）、Western blot、苏木素-伊红（HE）染色检测各组差异.结果 与对照组比较,模型组大鼠脊髓损伤后炎症纤维化显著,钙蛋白酶Ⅰ、GSK3β、Ⅰ型胶原（Collagen Ⅰ）、Ⅲ型胶原（CollagenⅢ）的表达均显著升高（0.92±0.04、0.91 ±0.06、1.19±0.02、1.07±0.03,P＜0.05）.应用蛋白酶抑制剂SJA6017干预后,大鼠脊髓瘢痕面积缩小[对照组比模型组（3.37 ±0.04） mm2比（1.13±0.01） mm2,P＜0.05]、损伤部位炎症因子[对照组比模型组（350±41） ng/L比（46 ±7） ng/L,P＜0.05]及纤维化蛋白表达[对照组比模型组（290±16） μg/L比（19 ±3） μg/L,P＜0.05]改善,GSK3β表达降低为0.42 ±0.02和0.35 ±0.03（P ＜0.05）.结论 钙蛋白酶Ⅰ通过GSK3β对脊髓损伤后炎症纤维化的调节发挥作用.Objective To evaluate the effects of Calpain Ⅰ and glycogen synthase kinase 3β （GSK3β） on chronic fibrosis following spinal cord injury.Methods Twenty Sprague-Dawley rats were randomized into control group,and model group.Reverse transcriptase-polymerase chain reaction （RT-PCR）,Western blotting,and hematoxylin and eosin staining were used to determine the difference between the groups.Results As compared with control group,chronic cystitis with diffuse fibrosis in model group was aggravated,and the levels of Calpain Ⅰ,GSK3 β,Collagen Ⅰ and Collagen Ⅲ were significantly increased （0.92 ± 0.04,0.91 ± 0.06,1.19 ± 0.02,1.07 ± 0.03,P 〈 0.05）.After administration of two doses of SJA6017,the scar area in the model group was decreased significantly,while the inflammation [control vs.model：（3.37 ±0.04） mm2 vs.（1.13 ±0.01） mm2,P〈0.05],fibrosis factors （control vs.model：（350 ±41） ng/L vs.（46 ±7） ng/L,P〈0.05] and expression of the fibrosis proteins [control vs.model：（29 ±16） μg/L vs.（19 ±3） μg/L,P〈0.05] all reduced.And the expression of GSK3β was reduced to 0.42 ±0.02 and 0.35 ±0.03 respectively （P〈0.05）.Conclusion Calpain Ⅰ and GSK3β may have an effect on the chronic fibrosis after spinal cord injury.

关 键 词：脊髓损伤 纤维化 钙蛋白酶Ⅰ 糖原合成酶激酶3B 炎症 

分 类 号：R651.2[医药卫生—外科学]