精囊凝胶蛋白Ⅰ低表达与慢性精囊炎的相关性研究  

Relationship between low semenogelin Ⅰ expression in human seminal vesicle and seminal vesiculitis

作　　者：宋震[1] 刘边疆[1] 崔洪青[1] 房建正[1] 邓云飞[1] 张胜利[1] 王海南[1] 苏仕峰[1] 章轶[1] 张炜[1] 王增军[1] 

机构地区：[1]南京医科大学第一附属医院江苏省人民医院泌尿外科,210029

出　　处：《中华实验外科杂志》2015年第3期647-649,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81270685）

摘　　要：目的 探讨精囊凝胶蛋白Ⅰ （SgⅠ）与慢性复发性精囊炎的关系,分析慢性精囊炎发生发展的分子机制.方法 采用反转录-聚合酶链反应（RT-PCR）、免疫组织化学及Western blot技术分别检测10例正常精囊组织及28例慢性复发性精囊炎组织中Sg Ⅰ mRNA、蛋白表达差异.结果 RT-PCR结果显示,8例精囊炎组织中Sg Ⅰ mRNA水平显著低于正常精囊组织,免疫组织化学法及Western blot结果示分别有83.3％ （10/12）及75.0％（6/8）的精囊炎组织中Sg Ⅰ蛋白为低表达.结论 SgⅠ低表达在慢性精囊炎的发生发展中起重要作用.Objective To investigate the relationship between semenogelin Ⅰ （Sg Ⅰ） expression and seminal vesiculitis,and the possible molecular mechanism of seminal vesiculitis.Methods Reverse transcription-polymerase chain reaction （RT-PCR）,immunohistochemistry and Western blotting were respectively performed to explore Sg Ⅰ mRNA and protein expression differences between 10 cases of normal seminal vesicle and 28 cases of chronic seminal vesiclitis.Results RT-PCR showed that Sg Ⅰ mRNA level in 8 cases of seminal vesiculitis was significantly lower than that in control group.Immunohistochemistry and Western blotting resulted in 83.3% （10/12） and 75.0% （6/8） of low Sg Ⅰ expression in seminal vesiculitis samples.Conclusion The low expression of Sg Ⅰ plays a vital role during the development of seminal vesilculitis.

关 键 词：精囊炎 精囊凝胶蛋白Ⅰ 精囊镜 抗菌活性 

分 类 号：R697.42[医药卫生—泌尿科学]