埃博霉素-菌蜕系统抑制肿瘤细胞增殖作用  

Inhibition of Epothilone-bacterial ghost systems on proliferation of tumor cells

作　　者：秦春晶 祝文兴[1] 刘新利[1] 

机构地区：[1]齐鲁工业大学生物工程学院,山东济南250353

出　　处：《济南大学学报（自然科学版）》2015年第1期26-29,共4页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金(21272139);国家高技术研究发展计划(863)(2006AA09Z415);山东省科技发展计划(2012GSF12107)

摘　　要：利用大肠杆菌菌蜕搭载埃博霉素B(BG-Epo B),研究其对Hela细胞增殖的抑制作用,并且对搭载条件进行优化。MTT实验结果显示,BG-Epo B对Hela细胞的毒性作用显著大于游离埃博霉素B(Epo B,p<0.05),IC50值为8.2 mg/L,透射电镜观察发现,BG-Epo B对Hela细胞的杀伤力增强。E. coli BG loading epothilone B（ BG- Epo B） was used to investigate their impact on the proliferation of Hela cells.The cytotoxic activity of the drug is determined by MTT tests and the results show that the application of BG-Epo B could significantly reduce the cell viability. The IC50 value of BG-Epo B on Hela cells is 8. 2 mg / L,and TEM observation proves that BG-Epo B could cause more harm on Hela cells than free Epo B.

关 键 词：埃博霉素B 菌蜕 HELA细胞 药物递送载体 

分 类 号：Q291[生物学—细胞生物学]