间充质干细胞移植对烧伤大鼠的炎症调节作用初探  

Immunosuppression effects of MSCs transplantation in rat burn models

作　　者：张进进[1,2] 范力星[2] 余元伦[2] 黄永莉[2] 邢颜超[2] 

机构地区：[1]新疆医科大学基础医学院,乌鲁木齐830011 [2] 兰州军区乌鲁木齐总医院输血科,乌鲁木齐830000

出　　处：《国际生物医学工程杂志》2015年第1期19-24,I0005,共7页International Journal of Biomedical Engineering

基　　金：兰州军区科研基金资助项目（CLZ13JB15）

摘　　要：目的 检测比较间充质干细胞（MSCs）移植治疗对烧伤大鼠炎症相关指标白细胞（WBC）、C反应蛋白（CRP）、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）、白介素-6（IL-6）、白介素-10（IL-10）水平变化的影响,探讨MSCs移植治疗烧伤的抑炎机制、效果及临床意义.方法 取正常新生儿脐带,分离脐带华通氏胶,培养脐带MSCs.另取体质量为（200±5）g SD雄性大鼠制备烧伤模型并随机分为对照组和移植组,移植组在烧伤24 h后进行皮下注射MSCs移植（2×10^6个/只）.分别于烫伤后0、1、2、3、5、7d采集血样,检测其WBC、CRP及炎症因子IFN-γ、TNF-α、IL-6、IL-10含量,并比较7、14、21、28 d时2组间创面愈合率及创面愈合时间.结果 对照组WBC烧伤后1、2d显著升高,3d后逐渐降低,移植组仅出现轻微升高;CRP水平变化比较,对照组伤后2、3d出现较大幅度上升,移植组变化甚微;血清中的炎症因子TNF-α、IL-6和IL-10比较,对照组和移植组于伤后3、5、7d均有增高,其中对照组增高更为明显;IFN-γ比较2组间未见明显差别.MSCs移植组14、21、28 d创面愈合率及创面愈合时间均明显优于对照组.结论 MSCs移植治疗烧伤大鼠,能明显减少烧伤大鼠血清中WBC、CRP、TNF-α、IL-6、IL-10的含量,减轻了由细胞炎症因子介导的炎症反应,但MSCs移植并未影响IFN-γ的产生.MSCs移植可通过降低烧伤后炎症因子产生而抑制其继发性炎症反应,从而促进创面愈合及烫伤修复.Objective To explore the immunosuppression effects,outcomes and clinical significance of mesenchymal stem cells （MSCs） transplantation in burn treatment by comparing the levels of WBC,C-reaction protein （CRP）,interferon-γ（IFN-γ）,interferon-o （TNF-α）,interleukin-6 （IL-6）,interleukin-10 （IL-10） between control burn models and models conducting MSCs transplantation.Methods After stripping Wharton＆#39;s Jelly from the neonatal umbilical cord,MSCs was cultured and expanded.Burn models were constructed in male SD rats weighted at （200± 5） g,and randomly grouped for control and MSCs transplantation.The rats in transplantation group were injected subcutaneously with hUCMSCs （1.5× 10^6） at 24 h after burning.The content of WBC,CRP,IFN-γ,TNF-α,IL-6,IL-10 in blood samples at 0,1,2,3,5 and 7 d after burning were detected by enzyme-linked immunosorbent assay （ELISA）.The ELISA results,the wound healing rate at 7,14,21 and 28 d,as well as wound healing time were compared and analyzed statistically by ANOVA between the two groups.Results WBC in control group increased significantly at 1 and 2 d,and CRP in control group increased substantially at 2 and 3 d.IFN-% IL-6 and IL-10 levels in serum showed increase on 5 d,and TNF-α arrived at its peak value at 7 d.By contrast,WBC,CRP,TNF-α,IL-6 and IL-10 of the MSCs transplantation group showed slightly increase after burning and the differences were convinced by statistical analysis,while IFN-γ showed little difference among the two groups.The MSCs transplantation group also showed significantly higher wound healing rate at 14,21,28 d and shorter wound healing time than that of the control.Conclusions MSCs transplantation can suppress the over-inflammatory response by significantly mediating the inflammatory cytokines as WBC,CRP,TNF-α,IL-6,IL-10 in burn.IFN-γseems not affected by MSCs transplantation.MSCs would be suitable to promote wound healing and repair in burn,which is achieved not only by differentiation and paracrine,but also by

关 键 词：间充质干细胞 细胞移植 烧伤 炎症因子 免疫调节 

分 类 号：Q813[生物学—生物工程] R644[医药卫生—外科学]