大鼠棉花烟雾吸入性肺损伤中的氧化应激  

Oxidative stress in cotton smoke inhalation-induced acute lung injury in rats

作　　者：姜毅[1,2] 韩志海[3] 黄燕[1] 王晓阳[1] 方庭正[1] 段蕴铀[1] 

机构地区：[1]第二军医大学海军临床医学院,北京100048 [2]沈阳军区政治部门诊部,110032 [3]海军总医院呼吸内科,北京100048

出　　处：《国际呼吸杂志》2015年第6期436-439,共4页International Journal of Respiration

基　　金：全军医学科研“十二五”计划科研课题（CWS11J180）

摘　　要：目的探讨大鼠棉花烟雾吸入性肺损伤中的氧化应激反应机制。方法18只雄性SD大鼠随机分成对照组、6h组、24h组，每组6只。复制大鼠棉花烟雾吸入性肺损伤模型，6h组、24h组大鼠分别在烟雾吸入后6h或24h行安乐死，ELISA法检测大鼠肺组织丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）、γ-谷氨酰半胱氨酸合成酶（γ—GCS）浓度，荧光定量PCR法行肺组织iNOS mRNA、γ-GCS mRNA定量。结果6h组、24h组大鼠肺组织匀浆中的MDA、NO、iNOS、YGCS浓度、iNOS mRNA和γ-GCS mRNA的相对表达量均高于对照组，且NO浓度、iNOS mRNA和γ-GCSmRNA的相对表达量24h组高于6h组（P值均〈0．05）；而6h组和24h组GSH浓度均低于对照组，且24h组低于6h组（P值均〈0．05）。结论在大鼠棉花烟雾吸入性肺损伤中，氧化应激反应加剧，同时抗氧化体系的激活不足以对抗氧化应激损伤，氧化／抗氧化体系失衡，导致肺损伤逐渐加重。Objective To investigate the mechanisms of oxidative stress in cotton smoke inhalationinduced acute lung injury in rats. Methods Eighteen male SD rats were randomly divided into control group,6 h group,and 24 h group. The rat model of cotton smoke inhalation injury was established. After smoke inhalation rats were mercy killed. The concentrations of malondialdehyde （MDA）, glutathione （GSH） ,nitric oxide （NO）, inducible nitric oxide synthase （iNOS）, and γ-glutamyl systeine synthetase （γ-GCS） in homogenized lung tissue were measured by enzyme-linked immunosorhent assay and the relative expressions of iNOS mRNA and γ-GCS mRNA were detected by fluorescence quantitative PCR. Results Compared with the control group, concentrations of MDA, NO, iNOS, γ-GCS and relative expressions of iNOS mRNA and γ-GCS mRNA in homogenized lung tissue in 6 h group and 24 h group were significantly elevated （all P 〈 0.05）, moreover, concentration of NO and relative expressions of iNOS mRNA and γ-GCS mRNA in 24 h group were higher than those in 6 h group （all P 〈0.05）. The concentration of GSH in homogenized lung tissue in 6 h group was lower than that in control group,but higher than that in 24 h group. Conclusions Oxidative stress significantly enhances in rats with cotton smoke inhalation-induced acute lung injury, meanwhile, the activation of antioxidative system is not enough. As a result,the oxidative-antioxidative balance is disturbed and lung injury becomes worse.

关 键 词：急性肺损伤 烟雾吸入性损伤 氧化应激 

分 类 号：R563[医药卫生—呼吸系统]