生理体液法测定40例健康志愿者血浆游离氨基酸正常值  

Establishing Physiological Body Fluid Method to Evaluate the Normal Value of Plasmic Free Amino Acid in Normal Healthy Volunteers

作　　者：何桂珍[1] 陶智兵 张文辉 王洁'[1] 

机构地区：[1]北京协和医院肠外肠内营养科,北京100730 [2]北京苏里曼制药有限公司,北京102127

出　　处：《现代检验医学杂志》2015年第1期46-51,54,共7页Journal of Modern Laboratory Medicine

基　　金：本研究获国家自然科学基金（30940069）和北京市自然科学基金（7102127）资助.

摘　　要：目的：建立生理体液的方法分析血浆氨基酸和测定健康志愿者血浆游离氨基酸正常值。方法①建立方法学：包括血样重复性、回收率、线性、稳定性；②测定40例健康志愿者血浆氨基酸正常值。结果不同浓度的标准液（2 nmol，4 nmol和8 nmol）进行分析，以氨基酸的峰面积对进样量作线性回归，所有氨基酸的R2均在1-0.999；血样不同氨基酸的批内重复性（rsd：0.30-2.90）和批间重复性（rsd：0.40-5.16）；回收率93.1％-107.4％。测定40例健康志愿者（男∶女＝1∶1）的血浆氨基酸正常值，并与其它仪器测定的结果进行比较。结论用枸橼酸锂缓冲系统分析血样游离氨基酸，有很好的重复性、回收率、线性和稳定性。比较不同仪器测定的健康志愿者血浆游离氨基酸正常值表明有较好的一致性，证实生理液分析方法的可靠性。Objective To analyze the plasma amino acid levels and evaluate the free amino acids value of normal healthy volunteers in plasma by establishing physiological fluid method.Methods ①Establishment of methodology：including repeatability of blood sample,recovery rate,linearity,and stability;②The free amino acid values in plasma in 40 normal healthy volunteers were analyzed.Results With the standard solution of different concentrations （2,4 and 8 nmol）analyzed and linear regression of amino acid calculated,found that R2 of all the amino acids were within the range from 1 to 0.9 9 9.The repeatability with in batch and between batches of different amino acid in blood samples were respectively 0.30-2.90 （rsd）and 0.40-5.16（rsd）;recovery rates were 93.1%-107.4%.Then evaluated the normal values of plasma amino acid in 40 healthy volunteers （male vs female：1 vs 1 ）,and compared the results with that measured by other instruments.Conclusion With lithium citrate buffer system to analyze the free amino acid in blood samples,it displayed good reproducibility,recovery rate, linearity and stability.Comparison of different instruments measuring the free amino acids in plasma of normal healthy volunteers showed good agreement,confirming that the method of physiological fluid analysis is reliable.

关 键 词：氨基酸测定 氨基酸正常值 生理体液法 

分 类 号：R446.112[医药卫生—诊断学]