南天竹微卫星标记开发及特性分析（英文）  

Development and Characterization of Microsatellite Loci for Heavenly Bamboo(Nandina domestica)

作　　者：呼慧丽[1] 李波[1] 胡菀 孙志霞[1] 张志勇[1] 范邓妹[1] 

机构地区：[1]江西农业大学亚热带生物多样性实验室,南昌330045

出　　处：《植物分类与资源学报》2015年第2期141-144,共4页Plant Diversity

基　　金：National Natural Science Foundation of China(grant nos.31160043 and 31160082);National Science and Technology Support Program(2012BAC11B02)

摘　　要：南天竹（Nandina domestica）是一种具有较高观赏价值的常绿灌木。本研究从南天竹基因组中开发和筛选出10个微卫星位点,能在该物种中进行稳定PCR扩增且具一定的多态性。采用4个南天竹群体24个个体进行检测发现,每个位点的等位基因数为2-6,期望杂合度和表观杂合度分别为0.153-0.778和0-1。本研究结果为后续对南天竹遗传多样性的研究打下了基础。另外,这10个位点在小檗科4个其他物种中也有一定的通用性。We developed and characterized a total of 10 novel polymorphic microsatellite loci from the genome of an ornamental shrub,Nandina domestica. The number of alleles per locus in 24 individuals from four populations varied from 2 to 6. The expected and observed heterozygosity ranged from 0. 153 to 0. 778 and from 0 to 1,respectively.These markers will provide tools for future studies on population genetic diversity of N. domestica. Additionally,most of our new markers could yield the amplification products in four other Berberidaceae species.

关 键 词：南天竹 微卫星 遗传多样性 

分 类 号：Q943[生物学—植物学]