黄槐花粉生活力的快速测定及花粉采集最佳时期和方位研究  

Pollen Viability Rapid Test of Cassia surattensis and the Best Time and Side of Pollen Collection

作　　者：招礼军[1,2] 朱栗琼[1,2] 周振燕 黄秋容[1] 鲁尧[1] 刘威[1] 

机构地区：[1]广西大学林学院,广西南宁530004 [2]国家林业局中南速生材繁育重点实验室,广西南宁530004

出　　处：《黑龙江农业科学》2015年第3期67-69,共3页Heilongjiang Agricultural Sciences

基　　金：国家自然科学基金资助项目(31260093);广西自然科学基金资助项目(2013GXNSFAA019057)

摘　　要：为提高植物育种效率,用TTC染色法和HNO3检测法对黄槐不同方位盛花期与花蕾期的花粉生活力进行测定。结果表明:黄槐花蕾期的花粉无生活力;盛花期的花粉生活力用TTC染色法的测定结果显著高于HNO3检测法;不同方位间的花粉生活力存在极显著差异,不同方位的结实率也表现同样的趋势;黄槐花粉生活力及结实率在方位上的顺次为:东向>西向>南向>北向。因此,黄槐花粉生活力快速测定方法以TTC染色法为优,以花盛开时东向的花粉粒质量为佳。In order to improve the efficiency of plant breeding,the pollen viability of Cassia surattensis was tested by triphenyl tetrazolium chloride（TTC）straining method and nitric acid（HNO3）method in different florescence and sides.The results showed that the pollen of Cassia surattensis in alabastrum period was unviable.In flowering period,the pollen was viability tested by TTC staining method,which was obviously higher than that by HNO3 method tested.There were significant differences in pollen viability among different sides and the same tendency occurred in fruit sets.The pollen viability and fruit set of Cassia surattensis in different sides were：eastern〉western〉southern〉northern.The TTC straining method was the best rapidly test method for pollen viability of Cassia surattensis;and the pollen quality of plant eastern side in flowering was the best in pollen collecting.

关 键 词：黄槐 花粉生活力 快速测定 花期 方位 

分 类 号：S685.99[农业科学—观赏园艺]