发根农杆菌诱导产生发状根及其在植物科学领域中的应用  

Hairy Root Induced by Agrobacterium rhizogenes and the Application in Plant Science

作　　者：邵明成[1] 王大鹏[1] 

机构地区：[1]北京林业大学,北京100083

出　　处：《黑龙江农业科学》2015年第3期146-150,共5页Heilongjiang Agricultural Sciences

摘　　要：发根农杆菌Ri质粒上T-DNA中含有的rolB基因可以通过转化侵染植株形成大量的发状根。由于通过发状根体系容易获得再生的转化植株,而且这些植株可表现出很多能稳定遗传的表型变异,因此该技术在植物科学领域中有着广阔的应用前景。对发根农杆菌的生物学特性、Ri质粒的结构和功能、rolB基因的位点与特征、发根农杆菌诱导发状根的转化机制和方法、诱导发状根发根的影响因素、发状根除菌后植株再生及鉴定等方面进行了归纳和分析,并对发根农杆菌诱导产生发状根在植物科学各领域的应用进行了简单的综述。Agrobacterium rhizogenes contains Ri plasmid,the rolBgenes is on the T-DNA of Ri plasmid,rolB genes can form a lot of hairy root through the wound infection of Agrobacterium rhizogenes.By the transformation of hairy root can get regenerate plants which show many stabilizes in the phenotypic variation of the genetic,so it has a broad application prospect in plant genetic improvement.The biological characteristics of Agrobacterium rhizogenes and the structure and function of Ri plasmid were summarized,as well as rolBgene loci and characteristics,Agrobacterium rhizogenes transformation mechanism and the methods of the hairy root induction,the influence factors of inducing hairy root,identifying and hairy plantlet regeneration after the eradication of bacteria and so on.In addition,the application in plant sciences was reviewed.

关 键 词：发根农杆菌 RI质粒 ROLB基因 

分 类 号：S182[农业科学—农业基础科学]