过量表达琥珀酸输出蛋白SucE对谷氨酸棒杆菌厌氧生产琥珀酸的影响  

Effect of Overexpression of Succinate Exporter Suc E on Anaerobic Succinate Production by Corynebacterium glutamicum

作　　者：夏慧华[1] 尹国民 朱年青[1] 陈涛[1] 

机构地区：[1]天津大学化工学院,教育部系统生物工程重点实验室,天津300072 [2]烟台只楚药业有限公司,山东烟台264002

出　　处：《化学工业与工程》2015年第2期74-79,共6页Chemical Industry and Engineering

基　　金：国家重点基础研究发展计划(2011CBA00804;2012CB725203);天津市自然科学基金(12JCYBJC12900);教育部博士点基金(20100032120014)

摘　　要：谷氨酸棒杆菌Corynebacterium glutamicum ATCC 13032在厌氧条件下通过三羧酸循环还原臂合成琥珀酸。高效地将细胞内的琥珀酸输出到胞外,对琥珀酸的生物合成具有重要意义。本实验以C.glutamicum XZ为出发菌株过表达琥珀酸输出蛋白表达基因suc E。突变株C.glutamicum XZ(p Exhsuc E)的琥珀酸得率提高了7%,生产率提高了19%。通量分析表明,过表达suc E基因后乙醛酸循环的相对通量提高了50%。采用两阶段培养,突变株C.glutamicum XZ(p Exhsuc E)的琥珀酸产量达到518 mmol·L-1,厌氧阶段的比生产率为0.95 mmol g CDW-1·h-1,1 mol glucose的平均得率为1.5 mol。Corynebacterium glutamicum ATCC 13032 produces succinate through the reductive tricarboxylic acid cycle under anaerobic conditions. Efficient export of succinate into the culture supernatant is important to increase its production. In this study,suc E gene overexpression was introduced into strain C.glutamicum XZ. The resulting strain C. glutamicum XZ（ p Exhsuc E） showed a 7% increase in succinate yield and a 19% increase in succinate productivity. Also,the relative flux of the glyoxylate pathway increased by 50%. Using two-stage culture,succinate titer up to 518 mmol·L- 1was achieved with a specific productivity of 0. 95 mmol g CDW- 1·h- 1and a yield of 1. 5 mol（ mol glucose）- 1.

关 键 词：琥珀酸输出蛋白 代谢通量分析 两阶段发酵 谷氨酸棒杆菌 

分 类 号：Q819[生物学—生物工程]